Focal Adhesion Plaque Associated Cytoskeletons Are Involved in the Invasion and Metastasis of Human Colorectal Carcinoma
Focal Adhesion Plaque Associated Cytoskeletons Are Involved in the Invasion and Metastasis of Human Colorectal Carcinoma
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DOI：
10.3109/07357900903147184
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发表时间：
2009-09
影响因子：
2.4
通讯作者：
Hong-Jun Yang;Juan-zhi Chen;Wen-li Zhang;Yanqing Ding
中科院分区：
文献类型：
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作者：
Hong-Jun Yang;Juan-zhi Chen;Wen-li Zhang;Yanqing Ding
应用免疫荧光技术结合激光共聚焦扫描显微镜和荧光定量聚合酶链反应技术，对41例正常人结直肠粘膜、原发性结直肠腺癌和19例淋巴结转移灶中粘着斑相关细胞骨架talin、vinculin、paxillin和tensin的蛋白和mRNA表达进行了研究。所有标本均有表达。结果显示talin、vinculin、tensin和paxillin的表达与大肠癌的发生、浸润和转移有关。Talin、黏着斑蛋白和张力蛋白下调，而桩蛋白升高。因此，这些细胞骨架可能在大肠癌的发生发展过程中起双向调节作用。
ABSTRACT The protein and mRNA expression of focal adhesion plaque associated cytoskeletons, including talin, vinculin, paxillin, and tensin, was studied using immunofluorescence in combination with confocal laser scanning microscopy and fluorescent quantitative polymerase chain reaction in 41 matched samples of human normal colorectal mucosae, primary colorectal adenocarcinomas, and 19 separate lymph node metastases. All specimens showed expression. The results showed talin, vinculin, tensin, and paxillin expression were correlated with carcinogenesis, invasion, and metastasis of colorectal carcinoma (CRC). Talin, vinculin, and tensin underwent downregulation while paxillin went up. So these cytoskeletons may play bidirectional regulating roles during the progression of CRC.