Finding and Verifying Enhancers for Endothelial-Expressed Genes.
Finding and Verifying Enhancers for Endothelial-Expressed Genes.
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DOI：
10.1007/978-1-0716-2059-5_28
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发表时间：
2022
影响因子：
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通讯作者：
A. Neal;H. Rodriguez-Caro;S. De Val
中科院分区：
文献类型：
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作者：
A. Neal;H. Rodriguez-Caro;S. De Val
内皮表达基因增强子的鉴定和分析可以提供关于其上游转录调控因子的重要信息。然而，增强子鉴定可能具有挑战性，特别是对于生物信息学访问或经验有限的人，并且增强子活性模式的转基因分析可能过于昂贵。在这里，我们描述了如何使用UCSC基因组浏览器上显示的公开数据集来识别哺乳动物基因的假定内皮增强子。此外，我们详细介绍了如何利用镶嵌Tol2介导的转基因斑马鱼，以验证是否一个假定的增强子能够指导内皮细胞特异性的基因表达模式。
Identification and analysis of enhancers for endothelial-expressed genes can provide crucial information regarding their upstream transcriptional regulators. However, enhancer identification can be challenging, particularly for people with limited access or experience of bioinformatics, and transgenic analysis of enhancer activity patterns can be prohibitively expensive. Here we describe how to use publicly available datasets displayed on the UCSC Genome Browser to identify putative endothelial enhancers for mammalian genes. Furthermore, we detail how to utilize mosaic Tol2-mediated transgenesis in zebrafish to verify whether a putative enhancer is capable of directing endothelial-specific patterns of gene expression.