PRODUCTIVE INFECTION AND CELL-FREE TRANSMISSION OF HUMAN T-CELL LEUKEMIA-VIRUS IN A NON-LYMPHOID CELL-LINE
PRODUCTIVE INFECTION AND CELL-FREE TRANSMISSION OF HUMAN T-CELL LEUKEMIA-VIRUS IN A NON-LYMPHOID CELL-LINE
复制标题
DOI：
10.1126/science.6316502
复制
发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
WEISS, RA
中科院分区：
文献类型：
--
作者：
CLAPHAM, P;NAGY, K;WEISS, RA
人T细胞白血病病毒（HTLV），美国PL分离株，经共培养和无细胞接种，可传播到一个非淋巴样人成骨肉瘤（HOS）细胞系，命名为HOS/PL，但不传播到其它9个带有HTLV受体的细胞系。HOS和HOS/PL细胞不依赖白细胞介素2，也不表达抗Tac单克隆抗体识别的白细胞介素2受体。由日本MT 2细胞系释放的HTLV也传播到HOS细胞。感染的HOS细胞释放大量滴度的子代HTLV，其在抗原性上与亲本病毒无法区分，并且能够转化T细胞。
Human T-cell leukemia virus (HTLV), American PL isolate, was transmitted by cocultivation and by cell-free filtrates to a nonlymphoid human osteogenic sarcoma (HOS) cell line, designated HOS/PL, but not to 9 other lines bearing receptors for HTLV, HOS and HOS/PL cells are not dependent on interleukin-2 and do not express interleukin-2 receptors that are recognized by anti-Tac monoclonal antibody. HTLV released by the Japanese MT2 cell line was also transmitted to HOS cells. The infected HOS cells release substantial titers of progeny HTLV which is antigenically indistinguishable from parental virus and is able to transform T cells.