Tracking Single Molecules in Biomembranes: Is Seeing Always Believing?
Tracking Single Molecules in Biomembranes: Is Seeing Always Believing?
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DOI：
10.1021/acsnano.9b07445
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发表时间：
2019-10-01
期刊：
影响因子：
17.1
通讯作者：
Yu，Yan
中科院分区：
文献类型：
--
作者：
Yu，Yanqi;Li，Miao;Yu，Yan
细胞膜中分子的空间组织及其动态相互作用在调节细胞功能方面起着核心作用。单粒子跟踪(SPT)是一种对单个分子进行实时成像和跟踪的技术，它导致了关于细胞膜的这些时空复杂性的突破性发现。然而，出现了对可能对小组委员会结果产生误导性解释的因素的担忧。在这里，我们简要回顾了SPT在了解质膜纳米尺度异质性方面的应用，重点介绍了使用纳米粒子作为成像探针来跟踪细胞膜中单分子的动力学所面临的独特挑战、陷阱和限制。
The spatial organization of molecules in cell membranes and their dynamic interactions play a central role in regulating cell functions. Single-particle tracking (SPT), a technique in which single molecules are imaged and tracked in real time, has led to breakthrough discoveries regarding these spatiotemporal complexities of cell membranes. There are, however, emerging concerns about factors that might produce misleading interpretations of SPT results. Here, we briefly review the application of SPT to understanding the nanoscale heterogeneities of plasma membranes, with a focus on the unique challenges, pitfalls, and limitations that confront the use of nanoparticles as imaging probes for tracking the dynamics of single molecules in cell membranes.