Quantitatively Understanding Cellular Uptake of Gold Nanoparticles via Radioactivity Analysis.
Quantitatively Understanding Cellular Uptake of Gold Nanoparticles via Radioactivity Analysis.
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通过放射性分析定量了解金纳米粒子的细胞摄取。
DOI：
10.1166/jnn.2015.9486
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发表时间：
2015
影响因子：
--
通讯作者：
Wang,Xueding
中科院分区：
文献类型：
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作者：
Shao,Xia;Schnau,Paul;Qian,Wei;Wang,Xueding
多功能金纳米粒子（AuNPs）的发展在过去的二十年中经历了爆炸式的发展。然而，在AuNPs广泛应用于临床之前，关于详细的表面化学以及它们如何影响AuNPs在体内和体外的行为的许多问题仍然需要解决。在这项工作中，放射性分析用于定量评价I-125放射性标记的AuNP被癌细胞摄取。利用这种新方法，我们对飞秒激光消融产生的无表面活性剂的金纳米颗粒进行了初步的生物评价。已经定量了作为AuNP表面上的RGD密度的函数以及时间的函数的AuNP的细胞摄取。放射性分析可以揭示AuNPs与癌细胞的动态相互作用，并有助于实现AuNPs的优化设计，用于未来的临床应用。
The development of multifunctional gold nanoparticles (AuNPs) underwent an explosion in the last two decades. However, many questions regarding detailed surface chemistry and how they are affecting the behaviors of AuNPs in vivo and in vitro still need to be addressed before AuNPs can be widely adapted into clinical settings. In this work, radioactivity analysis was employed for quantitative evaluation of I-125 radiolabeled AuNPs uptakes by cancer cells. Facilitated with this new method, we have conducted initial bioevaluation of surfactant-free AuNPs produced by femtosecond laser ablation. Cellular uptake of AuNPs as a function of the RGD density on the AuNP surface, as well as a function of time, has been quantified. The radioactivity analysis may shed light on the dynamic interactions of AuNPs with cancer cells, and help achieve optimized designs of AuNPs for future clinical applications.