The effect of various types of inducing agents on hepatic microsomal monooxygenase activity in rainbow trout.
The effect of various types of inducing agents on hepatic microsomal monooxygenase activity in rainbow trout.
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DOI：
10.1016/0041-008x(81)90208-8
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发表时间：
1981-06
影响因子：
3.8
通讯作者：
M. Vodicnik;Clifford R. Elcombe;John J. Lech
中科院分区：
文献类型：
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作者：
M. Vodicnik;Clifford R. Elcombe;John J. Lech
不同类型的诱导剂对虹鳟鱼肝微粒体单加氧酶（MO）系统的影响进行了研究，并与3-甲基胆蒽或苯巴比妥型诱导剂预处理后观察到的诱导曲线进行了比较。2，3，7，8-四氯二苯并-对-二恶英（TCDD）可提高乙氧基香豆素-和乙氧基试卤灵-O-脱乙基酶活性（ECOD，EROD）以及细胞色素P-450含量，但对苯非他明-N-脱甲基（弯曲）无影响。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）的溶解微粒体从TCDD预处理的鱼表现出增强的蛋白质带的分子量为57000。虽然诱导的幅度不是很大，异黄樟素预处理虹鳟鱼导致类似的诱导曲线后观察到的TCDD管理这些动物。开蓬和灭蚁灵对任何测量参数都没有刺激作用，也没有引起溶解微粒体SDS-PAGE后蛋白质谱的变化。这些结果表明，鱼类不同的哺乳动物的肝微粒体MO系统的这些诱导剂的反应。
The effects of various types of inducing agents on the hepatic microsomal monooxygenase (MO) system of rainbow trout were examined and compared to the induction profiles observed following pretreatment with either 3-methylcholanthrene- or phenobarbital-type inducers. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) elevated ethoxycoumarin- and ethoxyresorufin-O-deethylase activities (ECOD, EROD) as well as the cytochrome(s) P-450 content, but had no effect on benzphetamine-N-demethylation (BeND). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of solubilized microsomes from TCDD-pretreated fish demonstrated the intensification of a protein band with a molecular weight of 57,000. Although the magnitude of induction was not as great, isosafrole pretreatment of rainbow trout resulted in an induction profile similar to that observed following TCDD administration to these animals. Neither Kepone nor mirex had a stimulatory effect on any of the measured parameters and neither caused a change in the protein profile following SDS-PAGE of solubilized microsomes. These results suggest that fish respond differently from mammals to these inducers of the hepatic microsomal MO system.