TEMPERATURE-GRADIENT PLATES FOR GROWTH OF MICROORGANISMS
TEMPERATURE-GRADIENT PLATES FOR GROWTH OF MICROORGANISMS
复制标题
用于微生物生长的温度梯度板
DOI：
10.1128/jb.83.3.463-469.1962
复制
发表时间：
1962
影响因子：
3.2
通讯作者：
T. S. Matney
中科院分区：
文献类型：
--
作者：
O. Landman;H. Bausum;T. S. Matney
作者：Otto E. (Fort Detrick，Frederick，MD），霍华德T. Bausum和托马斯S.马特尼用于微生物生长的温度梯度板。J. Bacteriol. 83：463-469。1962.-已经设计了不同的温度梯度板，用于通过连续温度范围研究固体介质上的微生物生长或在精细梯度温度下研究液体介质中的微生物生长。所有的板都是由厚规格的铝制成的;在一端提供的热量沿着金属的长度消散，从而产生梯度。梯度的形状和范围取决于所提供的热量、绝缘、环境温度和其他因素。可以检测到细菌菌株之间0.2 ℃的温度敏感性差异。该板易于构造和操作。铝的尺寸、温度测量模式和加热方法都可以修改，而不降低装置的基本实用性。在细菌生长的最高温度下，形成一个尖锐的生长前沿。在大多数菌株中，除了少数耐温突变体之外，所有在前端下方的细菌都形成菌落，并且所有在前端上方的细菌都被杀死。
Landman, Otto E. (Fort Detrick, Frederick, Md.), Howard T. Bausum, and Thomas S. Matney. Temperature-gradient plates for growth of microorganisms. J. Bacteriol. 83:463–469. 1962.—Different temperature-gradient plates have been devised for the study of microbial growth on solid media through continuous temperature ranges or in liquid media at finely graded temperatures. All plates are made of heavy-gauge aluminum; heat supplied at one end is dissipated along the length of the metal so that a gradient is produced. The shape and range of the gradient depends on the amount of heat supplied, the insulation, the ambient temperature, and other factors. Differences of 0.2 C in temperature sensitivity between bacterial strains can be detected. The plates are simple to construct and operate. The dimensions of the aluminum, the mode of temperature measurement, and the method of heating may all be modified without diminishing the basic utility of the device. A sharp growth front develops at the maximal temperature of growth of bacteria. In most strains, all bacteria below the front form colonies and all bacteria above the front are killed, except for a few temperature-resistant mutants.