Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces.
Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces.
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DOI：
10.1021/acsbiomaterials.0c00366.s001
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发表时间：
2020-07
影响因子：
5.8
通讯作者：
Sisi Liu;Xiaoan Wu;Yang Yu;X. Wen;Zhang Yu;Xi-Qiao Feng;Hucheng Zhao
中科院分区：
文献类型：
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作者：
Sisi Liu;Xiaoan Wu;Yang Yu;X. Wen;Zhang Yu;Xi-Qiao Feng;Hucheng Zhao
肿瘤干细胞(CSCs)在肿瘤转移过程中起着重要作用，是肿瘤异质性的重要原因。越来越多的证据表明，CSC的表型与肿瘤微环境有关。在这项研究中，我们报告了机械应力的梯度引导CD44和YAP在几何限制的多细胞薄片中的表达的空间模式。我们的研究表明，细胞骨架的收缩通过YAP移位到细胞核内来调节CD44的表达。结果表明，几何约束和机械应力是CSC空间构型的调节因素。这可能有助于了解肿瘤微环境与肿瘤发生的关系。
Cancer stem cells (CSCs) play a critical role in the cancer metastasis and account for tumor heterogeneity. Growing evidence indicates that the CSC phenotypes are related to the tumor microenvironment. In this study, we report that the gradient of mechanical stresses guides the spatial patterning of the expression of CD44 and Yes-associated protein (YAP) in the geometrically confined multicellular sheets. Our study shows that the cytoskeletal contraction regulates the expression of CD44 through the translocation of YAP into the nucleus. The results demonstrate that geometric confinement and mechanical stresses are the regulators in the spatial patterning of CSC. It may help to understand the relationship between the tumor microenvironment and oncogenesis.