Satellite-cell pool size does matter: Defining the myogenic potency of aging skeletal muscle
Satellite-cell pool size does matter: Defining the myogenic potency of aging skeletal muscle
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DOI：
10.1016/j.ydbio.2006.02.022
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发表时间：
2006-06-01
影响因子：
2.7
通讯作者：
Yablonka-Reuveni, Zipora
中科院分区：
文献类型：
--
作者：
Shefer, Gabi;Van de Mark, Daniel P.;Yablonka-Reuveni, Zipora
体内环境的恶化被认为是干细胞功能随着年龄的增长而下降的主要原因。干细胞支持组织维持的能力不仅取决于它们对周围环境的反应，还取决于它们的丰度。在这里，我们调查卫星细胞（肌源性干细胞）池的大小和它的潜力，通过老年参与肌肉维护。小鼠卫星细胞的数量和性能已经使用分子标记物进行了分析，所述分子标记物专门表征静止卫星细胞及其后代，因为它们通过增殖、分化和储备细胞的产生而过渡。这项研究证实，随着年龄的增长，快肌和慢肌的肌纤维中常驻卫星细胞的丰度下降。然而，卫星细胞固有的肌原性潜能并不随年龄而减弱。此外，老化的卫星细胞生态位保留了在FGR富集有丝分裂环境后支持有效肌生成的能力。总之，卫星细胞丰度，但不是肌生成潜力，随着年龄的增长而恶化。这项研究表明，在日常肌肉利用过程中参与肌纤维维持的卫星细胞群体在整个生命过程中没有得到充分补充。(c)2006年爱思唯尔公司All rights reserved.
The deteriorating in vivo environment is thought to play a major role in reduced stem cell function with age. The capacity of stem cells to support tissue maintenance depends not only on their response to cues from the surrounding niche, but also on their abundance. Here, we investigate satellite cell (myogenic stem cell) pool size and its potential to participate in muscle maintenance through old age. The numbers and performance of mouse satellite cells have been analyzed using molecular markers that exclusively characterize quiescent satellite cells and their progeny as they transit through proliferation, differentiation and generation of reserve cells. The study establishes that abundance of resident satellite cells declines with age in myofibers from both fast- and slow-twitch muscles. Nevertheless, the inherent myogenic potential of satellite cells does not diminish with age. Furthermore, the aging satellite cell niche retains the capacity to support effective myogenesis upon enrichment of the mitogenic milieu with FGR Altogether, satellite cell abundance, but not myogenic potential, deteriorates with age. This study suggests that the population of satellite cells that participate in myofiber maintenance during routine muscle utilization is not fully replenished throughout life. (c) 2006 Elsevier Inc. All rights reserved.