THE EFFECTS OF RESTRAINT OR HYPERTONIC SALINE STRESS ON CORTICOTROPIN-RELEASING FACTOR, ARGININE-VASOPRESSIN, AND PROENKEPHALIN-A MESSENGER-RNAS IS THE CFY, SPRAGUE-DAWLEY AND WISTAR STRAINS OF RAT
THE EFFECTS OF RESTRAINT OR HYPERTONIC SALINE STRESS ON CORTICOTROPIN-RELEASING FACTOR, ARGININE-VASOPRESSIN, AND PROENKEPHALIN-A MESSENGER-RNAS IS THE CFY, SPRAGUE-DAWLEY AND WISTAR STRAINS OF RAT
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DOI：
10.1016/0006-8993(94)91707-8
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发表时间：
1994-12-19
期刊：
影响因子：
2.9
通讯作者：
CHOWDREY, HS
中科院分区：
文献类型：
--
作者：
HARBUZ, MS;JESSOP, DS;CHOWDREY, HS
一般认为，不同品系的大鼠在急性应激后的应激反应是相同的。在本研究中，我们检查了Wister、Sprague-Dawley和CFY品系大鼠在暴露于束缚的主要心理应激或腹腔注射高渗盐水的身体应激后下丘脑-垂体-肾上腺轴的激活。我们研究了急性应激后室旁核（PVN）小细胞中促肾上腺皮质激素释放因子（CRF）和脑啡肽原A（PEA）mRNA以及PVN大细胞和小细胞区精氨酸加压素（AVP）的下丘脑激活。此外，我们还测量了皮质酮作为终点激活的指标。循环皮质酮和CRF mRNA增加，在所有三个菌株后，无论是压力。AVP和PEA mRNA增加后高渗盐水，但仅在CFY株后的限制。总体而言，CFY品系大鼠中测量参数的相对增加大于其他品系。这些数据表明，在三个品系的大鼠中，对急性应激的反应存在显著差异。在设计或解释任何研究应激反应的研究时，必须考虑到这些不同的反应。
It is generally assumed that the stress response of different strains of rat will be identical following exposure to acute stress. In the present study we have examined the activation of the hypothalamo-pituitary-adrenal axis in the Wister, Sprague-Dawley and CFY strains of rat following exposure to either the predominantly psychological stress of restraint or the physical stress of i.p. hypertonic saline injection. We have investigated the hypothalamic activation of corticotrophin-releasing factor (CRF) and proenkephalin A (PEA) mRNAs in the parvocellular cells of the paraventricular nucleus (PVN) and arginine vasopressin (AVP) in both the magnocellular and parvocellular regions in the PVN following acute stress. In addition we have measured corticosterone as an index of end-point activation. Circulating corticosterone and CRF mRNA were increased in all three strains following either stress. AVP and PEA mRNAs were increased following hypertonic saline but only in the CFY strain following restraint. Overall the relative increase in the parameters measured was greater in the CFY strain of rat than the other strains. These data demonstrate marked differences in response to acute stress in the three strains of rat examined. These varying responses must be taken into consideration when designing or interpreting any study investigating the stress response.