ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.
ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.
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探针是一种方便的工具,用于设计高度多重荧光原位杂交的探针。
DOI:
10.1038/s41598-020-76439-x
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发表时间:
2020-12-16
影响因子:
4.6
通讯作者:
Wang S
中科院分区:
文献类型:
--
作者:
Hu M;Yang B;Cheng Y;Radda JSD;Chen Y;Liu M;Wang S
Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.
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DOI:
10.1093/bioinformatics/btq033
发表时间:
2010-03-15
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
作者:
Quinlan AR;Hall IM
通讯作者:
Hall IM
DOI:
10.1126/science.aau5324
发表时间:
2018-11-16
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
Moffitt JR;Bambah-Mukku D;Eichhorn SW;Vaughn E;Shekhar K;Perez JD;Rubinstein ND;Hao J;Regev A;Dulac C;Zhuang X
通讯作者:
Zhuang X
影响因子:
16
作者:
Cardozo Gizzi, Andres M.;Cattoni, Diego, I;Nollmann, Marcelo
通讯作者:
Nollmann, Marcelo
DOI:
10.1073/pnas.1612826113
发表时间:
2016-09-27
影响因子:
11.1
作者:
Moffitt, Jeffrey R.;Hao, Junjie;Zhuang, Xiaowei
通讯作者:
Zhuang, Xiaowei
影响因子:
14.9
作者:
Rouillard, JM;Zuker, M;Gulari, E
通讯作者:
Gulari, E