ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.

ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.
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探针是一种方便的工具,用于设计高度多重荧光原位杂交的探针。

DOI:
10.1038/s41598-020-76439-x
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发表时间:
2020-12-16
期刊:
影响因子:
4.6
通讯作者:
Wang S
Wang S
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Hu M;Yang B;Cheng Y;Radda JSD;Chen Y;Liu M;Wang S

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荧光原位杂交(FISH)是一种显示特定基因组座位和RNA物种空间位置的有效方法。最近的技术进步利用FISH以高度多样化的方式可视化这些特征。值得注意的例子包括染色质示踪、RNA多路传输错误稳健FISH(MerFish)、核组结构的多路传输成像(MINA)和顺序单分子RNA FISH。然而,广泛采用这些方法的一个障碍是多路FISH探针设计的复杂性。在这篇文章中,我们介绍了一个易于使用的、多功能的、一体化的应用程序ProbeDealer,它可以为各种多路传输的FISH技术及其组合设计探针。ProbeDealer提供一站式商店,满足研究社区的多种鱼类设计需求。
Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.
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