Human virus-specific CD8+ CTL clones revert from CD45ROhigh to CD45RAhigh in vivo: CD45RAhighCD8+ T cells comprise both naive and memory cells.
Human virus-specific CD8+ CTL clones revert from CD45ROhigh to CD45RAhigh in vivo: CD45RAhighCD8+ T cells comprise both naive and memory cells.
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人病毒特异性 CD8 CTL 克隆在体内从 CD45ROhigh 恢复为 CD45RAhigh：CD45RAhighCD8 T 细胞包括初始细胞和记忆细胞。
DOI：
10.4049/jimmunol.162.12.7080
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发表时间：
1999
影响因子：
4.4
通讯作者：
J. Sissons
中科院分区：
文献类型：
--
作者：
M. Wills;Andrew J. Carmichael;M. Weekes;Kim Mynard;G. Okecha;R. Hicks;J. Sissons
人们普遍认为，人类CD 8+记忆细胞主要存在于CD 45 RO高群体中。在长期病毒携带者的PBMC中存在高频率的对人CMV被膜磷蛋白pp 65特异的CD 8+记忆CTL;对给定pp 65肽特异的大量记忆CTL包含已大大扩增的单个CTL克隆。在这项研究中，我们发现高频率的pp 65肽特异性记忆CTL前体的CD 45 RO高CD 45 RA-人口，但也有相当大的频率在CD 45 RA高亚群。因为PBMC中的大多数CD 8 + T细胞是CD 45 RA高，所以总pp 65特异性记忆CTL库中更多的是在CD 45 RA高区室中而不是在CD 45 RO高区室中。使用克隆型寡核苷酸探针定量体内单个pp 65特异性CTL克隆的大小，我们发现CD 45 RA高群体对CD 8+细胞中总病毒特异性克隆大小的贡献是CD 45 RO高群体的6至10倍。在原发性CMV感染期间，单个病毒特异性CTL克隆最初为CD 45 RO高，但在感染消退后，在CD 45 RO高和CD 45 RA高群体中均检测到该克隆。我们的结论是，CD 45 RA+人CD 8 + T细胞不仅包括幼稚细胞，但含有一个非常显着的比例的记忆细胞，它可以从CD 45 RO高恢复到CD 45 RA高表型在体内。
It has been generally believed that human CD8+ memory cells are principally found within the CD45ROhigh population. There are high frequencies of CD8+ memory CTL specific for the human CMV tegument phosphoprotein pp65 in PBMC of long-term virus carriers; the large population of memory CTL specific for a given pp65 peptide contains individual CTL clones that have greatly expanded. In this study, we found high frequencies of pp65 peptide-specific memory CTL precursors in the CD45ROhighCD45RA- population, but also appreciable frequencies in the CD45RAhigh subpopulation. Because the majority of CD8+ T cells in PBMC are CD45RAhigh, more of the total pp65-specific memory CTL pool is within the CD45RAhigh than in the CD45ROhigh compartment. Using clonotypic oligonucleotide probes to quantify the size of individual pp65-specific CTL clones in vivo, we found the CD45RAhigh population contributed 6- to 10-fold more than the CD45ROhigh population to the total virus-specific clone size in CD8+ cells. During primary CMV infection, an individual virus-specific CTL clone was initially CD45ROhigh, but after resolution of infection this clone was detected in both the CD45ROhigh and the CD45RAhigh populations. We conclude that CD45RA+ human CD8+ T cells do not solely comprise naive cells, but contain a very significant proportion of memory cells, which can revert from the CD45ROhigh to CD45RAhigh phenotype in vivo.
影响因子：
32.4
作者：
Murali-Krishna, K;Altman, JD;Ahmed, R
通讯作者：
Ahmed, R