Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes)
Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes)
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DOI：
10.1016/j.aquatox.2004.12.011
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发表时间：
2005-04-30
影响因子：
4.5
通讯作者：
Tominaga, N
中科院分区：
文献类型：
--
作者：
Yamaguchi, A;Ishibashi, H;Tominaga, N
为了在最短的时间内评价雌二醇-17β(E_2)、壬基酚(NP)、4-(1-金刚烷基)苯酚(ADP)、双酚A(BPA)、BPA代谢物4-甲基-2，4-二(4-羟基苯基)-1-烯(MBP)和4，4‘-二羟基-α-甲基二苯乙烯(DRMS)等雌激素类化合物的雌激素样活性，我们采用逆转录-聚合酶链式反应(RT-PCR)技术，研究了雌激素相关基因如卵黄原蛋白I、卵黄蛋白原II和α-型雌激素受体基因在青海稻种肝脏中的表达。这些雌激素反应基因在暴露8h后对选定的雌激素类化合物有较快的反应，肝脏卵黄蛋白原II和雌激素受体α基因的表达比卵黄蛋白原I基因的表达更敏感。结果表明，受试化合物的雌激素活性由高到低依次为E_2(100)、GT；MBP(0.38)、ADP(0.25)、GT；DHMS(0.05)、GT；NP(0.02)、GT；BPA(0.001)。此外，这项初步研究表明，ADP和DHMS应被认为是候选雌激素化合物，具有诱导雄性青竹肝脏雌激素反应基因的潜力。这些结果表明，用选定的雌激素化合物处理雄性青竹后，卵黄蛋白原1、卵黄蛋白原11和雌激素受体a基因的表达模式发生了变化，这些基因可能是在尽可能短的时间内筛选雌激素内分泌干扰物的有用的分子生物标志物。(C)2005 Elsevier B.V.保留所有权利。
To evaluate the estrogenic activities of selected estrogenic compounds such as estradiol-17 beta (E2), nonylphenol (NP), 4-(1-adamantyl)phenol (AdP), bisphenol A (BPA), BPA metabolite 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) and 4,4'-dihydroxy-alpha-methylstilbene (DRMS) in the shortest possible time, we investigated the expression of estrogen-responsive genes such as vitellogenin I, vitellogenin II and alpha-type estrogen receptor genes in the liver of male medaka (Oryzias latipes) using reverse transcription-polymerase chain reaction (RT-PCR) techniques. These estrogen-responsive genes responded rapidly to selected estrogenic compounds after 8 h exposure, and the expression of hepatic vitellogenin II and estrogen receptor a mRNA was found to be more responsive than that of vitellogenin I mRNA. As a result, the relative estrogenic potencies of tested chemicals descended in the order of E2 (100) > MBP (0.38) > AdP (0.25) > DHMS (0.05) > NP (0.02) > BPA (0.001). Moreover, this preliminary study indicates that AdP and DHMS should be considered as candidate estrogenic compounds with the potential to induce hepatic estrogen-responsive genes in male medaka. These results suggest that vitellogenin 1, vitellogenin 11 and estrogen receptor a gene expression patterns alter in male medaka treated with selected estrogenic compounds, and that these genes may be useful molecular biomarkers for screening estrogenic endocrine-disrupting chemicals in the shortest possible time. (c) 2005 Elsevier B.V. All rights reserved.