Genetic and phenotypic features of blood and genital viral populations of clinically asymptomatic and antiretroviral-treatment-naive clade A human immunodeficiency virus type 1-infected women
Genetic and phenotypic features of blood and genital viral populations of clinically asymptomatic and antiretroviral-treatment-naive clade A human immunodeficiency virus type 1-infected women
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DOI：
10.1128/jcm.00113-07
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发表时间：
2007-06-01
影响因子：
9.4
通讯作者：
Belec, Laurent
中科院分区：
文献类型：
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作者：
Andreoletti, Laurent;Skrabal, Katharma;Belec, Laurent
在这项研究中，我们评估了人类免疫缺陷病毒1型(HIV-1)基因区划是否与全身和生殖器病毒群体之间表型CCR5(R5)或CXCR4(X4)共受体使用差异有关。从274名非洲妇女队列中挑选了4名临床上没有症状和治疗不成熟的艾滋病毒-1感染者，因为他们没有所有已知的改变生殖道病毒产生动力学的生物辅助因素。从血浆和宫颈阴道分泌物(CVS)中扩增、亚克隆和测序HIV病毒的包膜序列(V1~V3)。通过生产重组病毒颗粒来确定CCR5或CXCR4共受体的使用，然后使用趋向性重组试验对指示细胞系进行单循环感染试验。在这四名入选的患者中，CVS来源的序列似乎与血液来源的序列在遗传上是不同的(P
In the present study, we assessed whether human immunodeficiency virus type 1 (HIV-1) genetic compartmentalization was associated with phenotypic CCR5 (R5) or CXCR4 (X4) coreceptor usage differences between the systemic and the genital viral populations. Four clinically asymptomatic and treatment-naive clade A HIV-1-infected patients were selected from a cohort of 274 African women, because they were free of all the biological cofactors known to modify the kinetics of viral production in the genital tract. HIV RNA envelope sequences (V1 to V3) derived from plasma and cervicovaginal secretions (CVS) were amplified, subcloned, and sequenced. CCR5 or CXCR4 coreceptor usage was determined by production of recombinant viral particles, followed by single-cycle infection assays of indicator cell lines, using the tropism recombinant test. In these four selected patients, CVS-derived sequences appeared to be genetically distinct from blood-derived sequences (P