In vitro development of gut-like tissue demonstrating rhythmic contractions from embryonic mouse intestinal cells
In vitro development of gut-like tissue demonstrating rhythmic contractions from embryonic mouse intestinal cells
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DOI：
10.1111/j.1365-2982.2006.00895.x
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发表时间：
2007-04-01
影响因子：
3.5
通讯作者：
Oishi, K.
中科院分区：
文献类型：
--
作者：
Ito-Dufros, Y.;Funakoshi, Y.;Oishi, K.
肠的节律性运动是由Cajal间质细胞（ICC）和肠神经系统调节的。节律性运动被认为是在胚胎后期间充质祖细胞向ICC分化后发生的。在这项研究中，我们成功地重建了肠样组织表现出有节奏的收缩，通过培养分散的细胞酶分离的小鼠肠在胚胎中期。将这些肠细胞重组成高密度的胶原凝胶，使其大量增殖，培养1周后长成肠样组织。重组后的组织显示有节奏的收缩，神经元和ICC、PGP9.5和c-Kit特异性标记蛋白染色阳性。该组织还通过神经细胞和ICC的发育显示出网络形成。此外，在硝苯地平存在下，c- kit免疫阳性细胞表现出自发的Ca2+振荡，这被认为与对应于慢波的电活动耦合。因此，该培养系统可能用于阐明胃肠运动的发育机制。
The rhythmic motility of the intestine is regulated by the interstitial cells of Cajal (ICC) and the enteric nervous system. Rhythmic motility is considered to occur after the differentiation of mesenchymal progenitor cells into ICC during the late embryonic period. In this study, we successfully reconstructed a gut-like tissue demonstrating rhythmic contractions by culturing dispersed cells enzymatically isolated from the mouse intestine during the mid-embryonic period. These intestinal cells were reconstituted into a collagen gel at high density, made to proliferate considerably, and grew into a gut-like tissue after 1 week of culturing. The reconstituted tissue showed rhythmic contractions and stained positive for the specific marker proteins of neurones and ICC, PGP9.5 and c-Kit. The tissue also demonstrated network formation by developing nerve cells and ICC. Moreover, in the presence of nifedipine, c-Kit-immunopositive cells showed spontaneous Ca2+ oscillation, which is considered to be coupled to the electrical activity that corresponds to slow waves. Therefore, this culture system may be of use in elucidating the developmental mechanisms of gastrointestinal motility.