Changes in CD23 expression of blood and skin in atopic eczema after Chinese herbal therapy
Changes in CD23 expression of blood and skin in atopic eczema after Chinese herbal therapy
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DOI：
10.1046/j.1365-2222.1998.00233.x
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发表时间：
1998-03-01
影响因子：
6.1
通讯作者：
Rustin, MHA
中科院分区：
文献类型：
--
作者：
Banerjee, P;Xu, XJ;Rustin, MHA
背景CD 23的异常表达已经证实了特应性湿疹患者外周血和皮损皮肤中单核细胞/巨噬细胞系列细胞上的IgE（低亲和力IgE受体），目前尚不清楚这种异常是由于这些患者单核细胞的基本系统性问题还是反映了皮肤组织内细胞群的局部变化。异常表达是由对成熟细胞的局部皮肤影响或单核细胞分化的基本变化引起的。这些畸变和临床严重程度之间的可能关系也进行了研究，通过重复这些免疫病理学研究后，一个疗程的有效治疗与中药therapeutic（CHT）。方法外周血单个核细胞获得过敏性湿疹患者治疗前，8周后。CHT的疗效根据临床情况进行量化。通过粘附于塑料分离单核细胞并培养长达7天。在培养的第2、5和7天收获样品并制备细胞离心涂片。在时间0时对单核细胞和来自培养物的成熟细胞进行免疫细胞化学染色以鉴定表型亚群。使用计算机图像分析设备定量该免疫细胞学，以确定巨噬细胞亚群的出现及其CD 23表达水平。结果异位性湿疹患者在治疗前和治疗后第0天表达CD 23的单核细胞数量增加，在治疗后第7天培养的单核细胞分化速度加快与正常对照组相比，培养时间。有效治疗不影响表达CD 23的外周血单核细胞的数量。然而，治疗确实导致皮肤中CD 23（+）成熟巨噬细胞数量的显著减少以及该部分表达水平的降低，这些结果表明，临床严重程度的变化与病变技能中成熟抗原呈递细胞上的CD 23表达更密切相关，而不是与外周血单核细胞CD 23表达的分化密切相关。提示病变皮肤内的局部因素支配成熟巨噬细胞上的CD 23的积累和表达，并且这些因素可能比可能全身发生的CD 23表达的畸变与疾病的发病机制更相关。
Background Aberrant expression of CD23 (low affinity IgE receptor) on cells of the monocyte/macrophage series in peripheral blood and lesional skin of patients with atopic eczema has been demonstrated, It is not known whether this abnormality results from a fundamental systemic problem of the monocytes of these patients or reflects local changes to cell populations within the skin tissues.Objectives This study was designed to determine whether this aberrant expression was caused by local cutaneous influences on mature cells or fundamental changes in monocyte differentiation. The possible relationship between these aberrations and clinical severity was also investigated by repeating these immunopathological studies after a course of efficacious treatment with Chinese herbal therapy (CHT).Methods Peripheral blood mononuclear cells were obtained from patients with atopic eczema before, and after 8 weeks of treatment. Efficacy of CHT was quantified on clinical grounds. Monocytes were isolated by adherence to plastic and cultured for up to 7 days. Samples were harvested at 2, 5 and 7 days of culture and cytospins prepared. Immunocytochemical staining to identify phenotypic subsets was performed on the monocytes at time 0 and on maturing cells from culture. This immunocytology was quantified using computerized image analysis equipment to determine the emergence of macrophage subsets and their level of CD23 expression. Biopsies were taken from lesional skin before and after treatment and immunohistology was performed on cryostat sections to determine the number of antigen presenting cells expressing CD23 as well as the level of expression of these molecules.Results The results showed that increased numbers of monocytes from patients with atopic eczema express CD23 at day 0 and that cultured monocytes from these patients differentiate faster during the 7 day culture period as compared to normal controls. Efficacious treatment did not affect the number of peripheral blood monocytes expressing CD23. However, treatment did lead to a significant decrease in the number of CD23(+) mature macrophages in the skin as well as a reduction in the level of expression of this moiety, These results demonstrate that changes in clinical severity are more closely related to the expression of CD23 on mature antigen presenting cells in lesional skill rather than to differentiating peripheral blood monocyte CD23 expression.Conclusions These results suggests that local factors within lesional skin govern the accumulation and the expression of CD23 on mature macrophages and that these factors may be more relevant to the pathogenesis of the disease than aberrations in CD23 expression that may occur systemically.