Phenotypic modulation of smooth muscle cells and vascular remodeling in intraparenchymal small cerebral arteries after canine experimental subarachnoid hemorrhage
Phenotypic modulation of smooth muscle cells and vascular remodeling in intraparenchymal small cerebral arteries after canine experimental subarachnoid hemorrhage
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DOI：
10.1016/s0304-3940(03)00464-6
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发表时间：
2003-07
影响因子：
2.5
通讯作者：
H. Ohkuma;Shigeharu Suzuki;K. Ogane
中科院分区：
文献类型：
--
作者：
H. Ohkuma;Shigeharu Suzuki;K. Ogane
脑微循环变化是调节脑血流的重要因素。本研究的目的是探讨蛛网膜下腔出血（SAH）后脑实质内小动脉平滑肌细胞（SMC）表型调节和血管重构的可能性。犬实验性SAH后7 ~ 14天，在肺实质穿通动脉中，Northern blot检测到β -actin mRNA数量增加，聚合酶链反应检测到β -actin mRNA 3 '非翻译区结构变化增强，免疫组化显示肌苷重链胚胎亚型明显诱导，同时平滑肌肌球蛋白重链（SM2）表达降低。组织学形态学分析显示动脉壁面积增加，但SMC核数不变。这是首次报道表明，伴随表型调节的血管重塑发生在实质内小动脉。这些变化可能通过诱导脑血管阻力增加而影响SAH后的脑血流。
Cerebral microcirculatory changes are an important factor regulating cerebral blood flow. The aim of this study was to investigate the possibility of phenotypic modulation of smooth muscle cell (SMC) and vascular remodeling of intraparenchymal small cerebral arteries after subarachnoid hemorrhage (SAH). Seven to 14 days after canine experimental SAH, in intraparenchymal perforating arteries, the amount of beta-actin mRNA evaluated by Northern blot analysis increased, the structural change of the 3′ untranslated region of beta-actin mRNA detected by polymerase chain reaction analysis was enhanced, and immunohistochemistry showed marked induction of the embryonal isoform of myosine heavy chain accompanied by decreased expression of smooth muscle myosin heavy chain (SM2). Histological morphometric analysis showed an increase in the area of the arterial wall without changes in the number of nuclei of SMC. This is the first report suggesting that vascular remodeling accompanied by phenotypic modulation occurs in intraparenchymal small arteries. These changes may affect cerebral blood flow after SAH by inducing increased cerebrovascular resistance.