Alpha-smooth muscle actin within epithelial islands is predictive of ameloblastic carcinoma
Alpha-smooth muscle actin within epithelial islands is predictive of ameloblastic carcinoma
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DOI：
10.1016/j.oraloncology.2008.11.011
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发表时间：
2009-09-01
期刊：
影响因子：
4.8
通讯作者：
Salo, T.
中科院分区：
文献类型：
--
作者：
Bello, I. O.;Alanen, K.;Salo, T.
成釉细胞瘤是最常见的具有临床意义的牙源性肿瘤。它被认为是良性的，但具有局部侵袭性，并与可变的临床病理行为相关。成釉细胞癌是一种除细胞学异型性外还具有成釉细胞瘤特征的恶性肿瘤，伴有或不伴有转移。它具有侵袭性并与不良预后相关。本研究的目的是检查哪些上皮和基质标记物可以预测组织学诊断的成釉细胞癌，并可以将其与实性/多囊性成釉细胞瘤（SA）充分区分。我们使用图像 (ICM) 和流式细胞术 (FCM) 对 3 个成釉细胞癌和多达 18 个 SA 中的免疫组织化学 Ki-67、上皮膜抗原 (EMA)、α-平滑肌肌动蛋白 (α-SMA)、钙调蛋白、p63 和 DNA 含量进行了检查。重要的发现是成釉细胞癌中Ki-67标记指数显着高于SA，而EMA、calponin、p63、ICM和FCM不能充分区分两组病变。 α-SMA 的表达始终在成釉细胞癌的上皮岛细胞内获得，而在 SA 中则不然，尽管该标记物在两种病变的基质中均得到良好表达。因此，我们得出结论，上皮岛内 α-SMA 的存在高度预测成釉细胞癌。 (C) 2008 Elsevier Ltd. 保留所有权利。
Ameloblastoma is the most common clinically significant odontogenic tumor. It is considered benign but locally invasive and associated with variable clinico-pathological behavior. Ameloblastic carcinoma is a malignant tumor having features of ameloblastoma in addition to cytologic atypia with or without metastasis. It is aggressive and associated with poor prognosis. The aim of this study was to examine which epithelial and stromal markers are predictive of histologically diagnosed ameloblastic carcinoma and can sufficiently differentiate it from solid/multicystic ameloblastoma (SA). We examined immunohistochemically Ki-67, epithelial membrane antigen (EMA), alpha-smooth muscle actin (alpha-SMA), calponin, p63 and DNA content using image (ICM) and flow cytometry (FCM) in three ameloblastic carcinomas and up to 18 SAs. The important findings were that Ki-67 labeling index was significantly higher in ameloblastic carcinoma than SA while EMA, calponin, p63, ICM and FCM did not sufficiently differentiate the two groups of lesions. Expression of alpha-SMA was consistently obtained within the epithelial island cells of ameloblastic carcinoma and not in SA, although the marker was well expressed in the stroma of both lesions. We therefore conclude that the presence of alpha-SMA within the epithelial islands is highly predictive of ameloblastic carcinoma. (C) 2008 Elsevier Ltd. All rights reserved.