Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.
Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.
复制标题
盘基网柄菌中的肌球蛋白轻链激酶 (MLCK) 基因破坏：MLCK-A 在胞质分裂中的作用以及多种 MLCK 的证据。
DOI：
10.1073/pnas.93.22.12321
复制
发表时间：
1996
影响因子：
11.1
通讯作者：
Spudich,JA
中科院分区：
文献类型：
--
作者：
Smith,JL;Silveira,LA;Spudich,JA
我们已经创造了一株缺乏钙/钙调蛋白非依赖性MLCK-A的网柄苔藓。这种菌株进行胞质分裂的效率低于野生型，这导致在悬浮培养时多核细胞的频率增加。然而，MLCK-A-细胞能够发育并封端交联的表面受体，这一过程需要肌球蛋白重链。MLCK-A-细胞中仍存在磷酸化调节轻链(RLC)，这表明Dictyostelius具有一个或多个额外的能够磷酸化RLC的蛋白激酶。刀豆蛋白A处理可诱导野生型细胞中几乎所有RLC的磷酸化，但MLCK-A-细胞中的RLC磷酸化水平不受刀豆蛋白A的影响。因此，MLCK-A是与细胞中的另一种MLCK(S)分开调节的。
We have created a strain of Dictyostelium that is deficient for the Ca2+/calmodulin-independent MLCK-A. This strain undergoes cytokinesis less efficiently than wild type, which results in an increased frequency of multinucleate cells when grown in suspension. The MLCK-A-cells are able, however, to undergo development and to cap crosslinked surface receptors, processes that require myosin heavy chain. Phosphorylated regulatory light chain (RLC) is still present in MLCK-A-cells, indicating that Dictyostelium has one or more additional protein kinases capable of phosphorylating RLC. Concanavalin A treatment was found to induce phosphorylation of essentially all of the RLC in wild-type cells, but RLC phosphorylation levels in MLCK-A-cells are unaffected by concanavalin A. Thus MLCK-A is regulated separately from the other MLCK(s) in the cell.