Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells
Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells
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DOI：
10.1007/s11033-010-0343-4
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Guan, Yongmei
中科院分区：
文献类型：
--
作者：
Liu, Haiying;Deng, Suo;Guan, Yongmei
Oct4是早期胚胎发生过程中多能性和维持胚胎干细胞和多能性细胞身份所必需的转录因子。miR-302是由8个microrna （mirna）组成的一组，在胚胎干细胞和多能细胞中特异性表达，对正常的多能细胞自我更新和多能性至关重要。但是，miR-302参与控制P19胚胎癌细胞自我更新和多能性的核心调控回路的机制尚未确定。在这里，我们发现Oct4是miR-302的表达和转录激活所必需的，并且Oct4结合到miR-302的假定启动子上，这表明Oct4激活了P19细胞中miR-302的初级转录本。本研究提出miR-302簇在P19细胞中作用于Oct4调控网络的下游。
Oct4 is a transcription factor that is required for pluripotency during early embryogenesis and the maintenance of embryonic stem (ES) cell and pluripotent cell identity. miR-302, a cluster of eight microRNAs (miRNAs) that are expressed specifically in ES cells and pluripotent cells, is crucial for normal pluripotent cell self-renewal and pluripotency. But, the mechanism by which miR-302 participates in the core regulatory circuitry that controls self-renewal and pluripotency in P19 embryonic carcinoma cells has not been established. Here, we show that Oct4 is required for the expression and transcriptional activation of miR-302 and that Oct4 binds to the putative promoter of miR-302, suggesting that Oct4 activates the primary miR-302 transcript in P19 cells. This study proposes that the miR-302 cluster acts downstream of the Oct4 regulation network in P19 cells.