Studies on absorption and metabolism of palatinose (isomaltulose) in rats
Studies on absorption and metabolism of palatinose (isomaltulose) in rats
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DOI：
10.1017/s0007114510003193
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发表时间：
2011-01-14
影响因子：
3.6
通讯作者：
Sasaki, Hajime
中科院分区：
文献类型：
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作者：
Tonouchi, Hidekazu;Yamaji, Taketo;Sasaki, Hajime
我们通过碳水化合物负荷试验和（13）C-和H（2）-呼吸试验评价了帕拉金糖在大鼠体内的吸收和代谢。我们将这些试验的结果与蔗糖的结果进行了比较，因为蔗糖是帕拉金糖的异构体，并且通常已知在小肠中降解和吸收。在碳水化合物负荷试验中，口服帕拉金糖后的血糖和血浆胰岛素水平逐渐升高，并达到低于蔗糖给药后的最大值。在（13）C-呼吸试验中，大鼠经口给予[1-（13）C]蔗糖或[1-（13）C]帕拉金糖，并饲养在一个室内。收集室内的呼出空气，在适当的时间间隔测量呼出气中的（13）CO（2）水平，持续360 min。从[1-（13）C（葡萄糖）]（[1-（13）C（glc）]）和[1-（13）C（glc）]到呼出气中的（13）CO（2）达到最大浓度所需的时间值[（fructose）]（[1-（13）C（fru）]）帕拉金糖分别显著长于[1-（13）C（glc）]和[1-（13）C（fru）]蔗糖。[1-（13）C（glc）]帕拉金糖的曲线下面积（AUC）值大于[1-（13）C（glc）]蔗糖的曲线下面积（AUC）值，但[1-（13）C（fru）]的AUC显示帕拉金糖和蔗糖之间无差异。在H（2）-呼气试验中，测定呼气中的H（2）浓度420 min。在帕拉金糖和蔗糖中几乎检测不到H（2），两组之间没有观察到显著差异。这些结果表明帕拉金糖在体内的利用率与蔗糖相等。
We evaluated the absorption and metabolism of palatinose in rats by the carbohydrate load test and the (13)C- and H(2)-breath tests. We compared the results of these tests with those of sucrose, since sucrose is an isomer of palatinose and generally known to be degraded and absorbed from the small intestine. In the carbohydrate load test, blood glucose and plasma insulin levels after oral administration of palatinose rose more gradually and reached a maximum that was lower than that after sucrose administration. In the (13)C-breath test, rats were orally administrated [1-(13)C]sucrose or [1-(13)C]palatinose and housed in a chamber. The expired air in the chamber was collected, and the level of (13)CO(2) in the expired air was measured at appropriate intervals for 360 min. The value of time taken to reach the maximum concentration for expired (13)CO(2) from [1-(13)C(glucose)] ([1-(13)C(glc)]) and [1-(13)C(fructose)] ([1-(13)C(fru)]) palatinose was significantly longer than that from [1-(13)C(glc)] and [1-(13)C(fru)]sucrose, respectively. The value of area under the curve (AUC) for [1-(13)C(glc)]palatinose was larger than that for [1-(13)C(glc)]sucrose, but AUC for [1-(13)C(fru)] showed no difference between palatinose and sucrose. In the H(2)-breath test, the concentration of H, in the expired air was measured for 420 min. H(2) was hardly detected with both palatinose and sucrose and no significant difference was observed between the two groups. These results suggest that palatinose is utilised in vivo at a rate equal to that of sucrose.