Evidence of parthenogenetic origin of ovarian teratoma:: Case report
Evidence of parthenogenetic origin of ovarian teratoma:: Case report
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DOI：
10.1093/humrep/deh345
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发表时间：
2004-08-01
影响因子：
6.1
通讯作者：
Nagy, ZP
中科院分区：
文献类型：
--
作者：
Oliveira, FG;Dozortsev, D;Nagy, ZP
本病例报告是为数不多的记录在案的孤雌胚胎从有卵巢畸胎瘤史的试管受精患者中取出的病例之一。一名29岁女性，因男性因素导致原发不育症6年。4年前，由于卵巢畸胎瘤，她接受了左侧卵巢切除术。在基础评估期间进行的超声扫描显示右侧卵巢有两幅复杂图像，提示畸胎瘤，尺寸分别为2.5 x 2.4和1.7 x 1.3 cm。超声检查显示卵巢实质明显正常，患者在IVF-ICSI周期中接受了重组卵泡刺激素刺激卵巢的醋酸leuprolide方案。她有13个中期II (MII)， 4个中期I (MI)， 2个生发囊泡（GV）卵母细胞和1个4细胞胚胎。9个注射的卵母细胞中有8个正常受精，1个未受精。取卵后72h进行胚胎移植。卵母细胞回收后的4细胞（孤雌）胚胎在培养中继续分裂，第二天发育成7细胞胚胎。胚形态正常，每个卵裂球有明显的细胞核。荧光原位杂交（FISH）对分析的每个卵裂球中的X染色体返回两个信号。在8个正常受精的胚胎中，有3个被移植，导致了正常的单胎妊娠和健康婴儿的出生。
This case report represents one of the few documented cases of parthenote embryo retrieval from an IVF patient with a history of ovarian teratomas. A 29-year-old woman presented at our centre with a history of primary infertility for 6 years due to male factor. She had undergone left oophorectomy 4 years before due to an ovarian teratoma. An ultrasound scan performed during basal evaluation revealed two complex images in the right ovary suggesting teratomas, measuring 2.5 x 2.4 and 1.7 x 1.3 cm. A significant extent of sonographically normal ovarian parenchyma was present, and the patient underwent the long leuprolide acetate protocol of ovarian stimulation with recombinant FSH for an IVF-ICSI cycle. She had 13 metaphase II (MII), four metaphase I (MI), two germinal vesicle (GV) oocytes and one 4-cell embryo retrieved. Eight out of nine injected oocytes were fertilized normally while one was unfertilized. Embryo transfer was carried out 72 h after retrieval. The 4-cell (parthenote) embryo recovered at oocyte retrieval continued to cleave in culture, developing into a 7-cell embryo by the next day. The embryo was morphologically normal, presenting an evident nucleus in each blastomere. Fluorescent in situ hybridization (FISH) returned two signals for the X chromosome in each blastomere that was analysed. Of the eight normally fertilized embryos, three were transferred, resulting in a normal singleton pregnancy and the birth of a healthy baby.