Selection and biologic properties of malignant variants of a murine lymphosarcoma.
Selection and biologic properties of malignant variants of a murine lymphosarcoma.
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鼠淋巴肉瘤恶性变体的选择和生物学特性。
DOI：
10.1093/jnci/61.6.1499
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
G. Nicolson
中科院分区：
文献类型：
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作者：
K. Brunson;G. Nicolson
开发了具有 RAW117 淋巴肉瘤细胞系的同基因肿瘤模型系统，用于研究与恶性状态增强相关的宿主和肿瘤细胞特性。该 BALB/c 小鼠模型与人类淋巴肉瘤相似，主要涉及肝脏和脾脏。通过静脉内接种肿瘤细胞、去除所得实体肝肿瘤结节用于体外生长、以及静脉内重新注射培养的细胞来进行连续体内选择。经过十次这样的肝脏植入、侵袭、存活和生长的连续选择后，获得了淋巴肉瘤细胞系（RAW 117-H10），在比较生物学测定中，该细胞系形成的肉眼肝脏肿瘤结节比亲本肿瘤细胞系多约200倍，并且当通过宿主死亡时间监测时，显示出增强的恶性特性。无论是亲代还是任何选定的 RAW117 细胞系，其他部位都很少出现肺结节或肿瘤，这证实了之前的报道，其中体内连续选择产生了在特定部位优先肿瘤定植的稳定细胞系。
A syngeneic tumor model system with the RAW117 lymphosarcoma cell line was developed for use in investigations of host and tumor cell properties associated with an enhanced state of malignancy. This BALB/c mouse model was found to be similar to human lymphosarcoma in that liver and spleen were the major organs involved. Sequential in vivo selections were performed by iv tumor cell inoculation, removal of resulting solid liver tumor nodules for growth in vitro, and reinjection of the cultured cells iv. After ten such sequential selections for implantation, invasion, survival, and growth in liver, a lymphosarcoma cell line was obtained (RAW 117-H10) that formed approximately 200-fold more gross liver tumor nodules than did the parental tumor cell line in comparative biologic assays and displayed enhanced malignant properties when monitored by time of host death. Very few lung nodules or tumors were present at other sites with either the parental or any of the selected RAW117 lines, which confirms previous reports in which in vivo sequential selection yielded stable cell lines with preferential tumor colonization at specific sites.