Properties and distribution of a lectin-like hemagglutinin differentially expressed by murine stromal tissue macrophages.
Properties and distribution of a lectin-like hemagglutinin differentially expressed by murine stromal tissue macrophages.
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DOI：
10.1084/jem.164.6.1862
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发表时间：
1986-12-01
影响因子：
15.3
通讯作者：
Gordon, S
中科院分区：
文献类型：
--
作者：
Crocker, P R;Gordon, S
我们描述了一种新的血凝素，这是差异表达的小鼠间质组织巨噬细胞。常驻骨髓巨噬细胞（RBMM）在体内与未成熟的、增殖的造血细胞物理上相关，与常驻腹膜巨噬细胞（RPM）不同，其在体外与未调理的绵羊红细胞（E）形成显著的红细胞。E的结合是巨噬细胞（M phi）特异性的，不伴随摄入，并且不依赖于温度（0-37 ℃）、二价阳离子和代谢抑制剂叠氮化物和碘乙酸盐。用胰蛋白酶预处理RBMM阻止了玫瑰花结的形成，但神经氨酸酶增强了玫瑰花结的形成。相反，如果用神经氨酸酶预处理E，则结合几乎被废除，而胰蛋白酶预处理配体导致轻微增强。E受体（SER）的凝集素样性质（对唾液酸化糖缀合物具有特异性）与我们观察到的神经氨酸乳糖或神经节苷脂GD 1a的结合抑制一致（分别在5-10 mM和11 μ M时抑制50%）。SER在新鲜分离的RBMM上的表达是异质的，并且与Ia抗原的表达呈现显著的负相关。在10%FCS中培养期间，RBMM上的SER水平下降，半衰期约为24小时。培养诱导的其他细胞表面变化包括Ia抗原表达的瞬时增加和Mac-1的获得。为了确定SER是否在其它间质M phi群体上表达，通过胶原酶消化或灌洗从各种组织分离贴壁细胞。E在RBMM和淋巴结间质M phi上的结合最高，在枯否细胞和脾间质M phi上处于中等水平，但在血液单核细胞和胸腺、腹膜、胸膜和支气管肺泡M phi上的结合较低或检测不到。因此，SER似乎在包埋在实体组织中的某些M phi群体上表达，但在通过灌洗回收的M phi中基本上不存在。它在单核细胞中的缺失意味着SER在进入组织后被M phi获得，在组织中它可以执行粘附功能。在骨髓中，RBMM上的SER可以与小鼠造血细胞上的适当唾液酸化配体相互作用，并且可以影响它们的生长和分化速率。
We describe a novel hemagglutinin which is differentially expressed on murine stromal tissue macrophages. Resident bone marrow macrophages (RBMM), which are physically associated with immature, proliferating hematopoietic cells in vivo, formed striking rosettes with unopsonized sheep erythrocytes (E) in vitro, unlike resident peritoneal macrophages (RPM). Binding of E was macrophage (M phi) specific, not accompanied by ingestion and independent of temperature (0-37 degrees C), divalent cations, and the metabolic inhibitors azide and iodoacetate. Pretreatment of RBMM with trypsin prevented rosette formation, but neuraminidase enhanced it. Conversely, binding was virtually abrogated if E were pretreated with neuraminidase, whereas trypsin pretreatment of the ligand resulted in a slight enhancement. The lectin-like nature of the E receptor (SER), with specificity for sialylated glycoconjugates, was consistent with the inhibition of binding we saw with neuraminyllactose or the ganglioside GD1a (50% inhibition at 5-10 mM and 11 microM, respectively). Expression of SER on freshly isolated RBMM was heterogeneous and exhibited a striking inverse correlation with expression of Ia antigens. During cultivation in 10% FCS, levels of SER on RBMM declined with a half-life of approximately 24 h. Other cell surface changes induced by cultivation included a transient increase in expression of Ia antigen and acquisition of Mac-1. To determine whether SER was expressed on other stromal M phi populations, adherent cells were isolated from various tissues by collagenase digestion or lavage. Binding of E was highest on RBMM and lymph node stromal M phi, at intermediate levels on Kupffer cells and splenic stromal M phi, but was low or undetectable on blood monocytes and thymic, peritoneal, pleural, and bronchoalveolar M phi. SER therefore appeared to be expressed on certain M phi populations embedded in solid tissues but was largely absent from M phi recoverable by lavage. Its absence from monocytes implies that SER is acquired by M phi after entering tissues where it may perform adhesive functions. In bone marrow, SER on RBMM could interact with an appropriate sialylated ligand on murine hematopoietic cells, and could influence their rate of growth and differentiation.