Fate of embryonic stem cells transplanted into the deafened mammalian cochlea
Fate of embryonic stem cells transplanted into the deafened mammalian cochlea
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DOI：
10.3727/000000006783981819
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发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Shepherd, R.
中科院分区：
文献类型：
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作者：
Coleman, B.;Hardman, J.;Shepherd, R.
螺旋神经节神经元（sgn）是耳蜗的初级传入神经元，在感觉神经性听力损失（SNHL）后，由于缺乏毛细胞正常接受的营养支持而退化。细胞移植正在成为内耳康复的一种潜在策略，因为注射细胞可能能够取代耳蜗中受损的sgn。存活的sgn数量的增加可能导致人工耳蜗（CIs）疗效的提高。我们检测了部分分化小鼠胚胎干细胞（MESCs）移植到耳聋豚鼠耳蜗后的存活率（n = 15）。通过圆窗将细胞通过显微注射直接送入左侧鼓室。在移植后的4周内，在鼓膜中检测到少量MESCs，其中一部分细胞在体内保留了68 kDa的神经丝蛋白表达。虽然这种递送方法需要改进以有效地长期替代受损的sgn，但少量MESCs能够在失聪的哺乳动物耳蜗中存活长达4周，而不会引起炎症组织反应。
Spiral ganglion neurons (SGNs), the primary afferent neurons of the cochlea, degenerate following a sensorineural hearing loss (SNHL) due to lack of trophic support normally received from hair cells. Cell transplantation is emerging as a potential strategy for inner ear rehabilitation, as injected cells may be able to replace damaged SGNs in the deafened cochlea. An increase in the number of surviving SGNs may result in improved efficacy of cochlear implants (CIs). We examined the survival of partially differentiated mouse embryonic stem cells (MESCs), following xenograft transplantation into the deafened guinea pig cochlea (n = 15). Cells were delivered directly into the left scala tympani via microinjection through the round window. Small numbers of MESCs were detected in the scala tympani for up to 4 weeks following transplantation and a proportion of these cells retained expression of neurofilament protein 68 kDa in vivo. While this delivery method requires refinement for effective long-term replacement of damaged SGNs, small numbers of MESCs were capable of survival in the deafened mammalian cochlea for up to 4 weeks, without causing an inflammatory tissue response.