Effect of the Chinese traditional prescription Suo Quan Wan on TRPV1 expression in the bladder of rats with bladder outlet obstruction.
Effect of the Chinese traditional prescription Suo Quan Wan on TRPV1 expression in the bladder of rats with bladder outlet obstruction.
复制标题
中药锁泉丸对膀胱出口梗阻大鼠膀胱TRPV1表达的影响
DOI：
10.1186/s12906-015-0898-7
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发表时间：
2015-12-01
影响因子：
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通讯作者：
Cao H
中科院分区：
文献类型：
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作者：
Lai H;Tan B;Liang Z;Yan Q;Lian Q;Wu Q;Huang P;Cao H
缩泉丸是一种治疗下尿路症状的中药方剂，在临床上已有数百年的应用历史。然而，SQW的功效和作用机制仍需科学依据。本研究探讨了SQW对膀胱出口梗阻（BOO）大鼠膀胱功能和膀胱瞬时受体电位香草酸1（TRPV 1）表达的影响。本研究通过观察膀胱过度活动症（overactive bladder，OAB）大鼠模型在出口梗阻不同时间段后膀胱功能的变化，以获得合适的大鼠模型。在第一部分中，雌性Sprague-Dawley大鼠接受假手术或部分BOO手术。分别于造模后第2、4、6周对模型组和对照组进行尿动力学检查，以测定膀胱功能的差异。一旦获得适当的大鼠模型，就进行实验的第二部分。造模后给予SQW治疗。进行尿动力学评估，然后切除大鼠膀胱。免疫荧光染色，实时定量PCR和Western blot进行定位和定量的表达TRPV 1在膀胱。结果第一部分的结果表明，在2和4周，OAB模型组表现出显着差异的尿动力学参数，包括膀胱压力，最大排尿压力，最大膀胱容量，与假手术组相比。造模后4周和6周，OAB模型组的残尿量（RV）和非排尿性收缩频率有显著性差异。与假手术6周组、OAB模型2周组和4周组相比，OAB模型6周组RV明显增加，但排尿效率明显降低。接受BOO的大鼠在4周前表现出与代偿状态相似的特征，并可能在6周时进入失代偿状态。采用4周OAB模型进行研究是合适的，实验二部分中，OAB模型组不稳定膀胱经SQW治疗后恢复膀胱稳定，膀胱肥大得到改善，尿动力学参数得到校正。膀胱组织TRPV 1 mRNA和蛋白表达在OAB模型组明显高于对照组，SQW治疗后膀胱组织TRPV 1 mRNA和蛋白表达明显降低。结论SQW可调节TRPV 1的表达，促进膀胱功能的恢复。
BackgroundSuo Quan Wan (SQW) is a Chinese traditional prescription that has been used in clinical treatment of lower urinary tract symptoms for centuries. However, scientific basis of SQW efficacy and mechanism is still needed. This study investigated the effect of SQW on bladder function and transient receptor potential vanilloid 1 (TRPV1) expression in the bladder of rats with bladder outlet obstruction (BOO). The induced changes in bladder function in overactive bladder (OAB) rat model were observed following different periods of outlet obstruction to obtain an appropriate rat model.MethodsThis study was carried out in two parts. In the first part, female Sprague–Dawley rats received sham operations or partial BOO operations. Two, four, and six weeks later, the OAB model groups and control were subjected to urodynamic tests to measure differences in bladder functions. Once the appropriate rat model was obtained, the second part of the experiment was performed. The rat model was recreated and treated with SQW. Urodynamic assessment was conducted, and the bladders of the rats were then removed. Immunofluorescence staining, real-time PCR, and Western blot were performed to localize and quantify the expression of TRPV1 in the bladder.ResultsResults of the first part indicated that at 2 and 4 weeks, the OAB model group exhibited significant differences in urodynamic parameters, including bladder pressure, maximum voiding pressure, and maximum bladder capacity, compared with the sham group. At 4 and 6 weeks, the OAB model group exhibited significant differences in residual volume (RV) and non-voiding contraction frequency. Six-week OAB model group showed much more RV but less voiding efficiency when compared with 6-week sham group or 2—and 4-week OAB model group. Rats that underwent BOO exhibited similarities with the compensated state before four weeks and may have entered decompensated state at six weeks. Studies conducted with 4-week OAB model were appropriate.In part two of the experiment, unstable bladder in the OAB model group recovered bladder stability after SQW treatment, accompanied by improved bladder hypertrophy, as well as corrected urodynamic parameters. Expression of TRPV1 mRNA and proteins in the bladder was significantly greater in the OAB model group than that in the control group, which subsequently decreased significantly with SQW treatment in BOO-induced rats.ConclusionsSQW can modulate the expression of TRPV1 in accordance with the recovery of bladder function.