Cell death during germ band inversion, dorsal closure, and nervous system development in the spider Cupiennius salei
Cell death during germ band inversion, dorsal closure, and nervous system development in the spider Cupiennius salei
复制标题
蜘蛛 Cupiennius salei 种带倒转、背侧闭合和神经系统发育过程中的细胞死亡
作者：
Nikola;W. Damen
细胞死亡是动物发育过程中的一个重要特征，因为它是胚胎控制发育组织中细胞数量从而决定生长器官形式的一种可能性。在果蝇中，细胞死亡已被证明与胚胎许多不同器官的形成有关。我们使用末端脱氧核苷酸转移酶介导的dUTP -二氧合蛋白缺口末端标记（TUNEL）和一种针对caspase - 3活化形式的抗体来可视化Cupiennius salei蜘蛛的细胞死亡。我们发现，与果蝇相似，神经系统的发育过程中也会发生大量细胞死亡，这表明这是节肢动物祖先的特征。我们还发现在腿部发育过程中细胞死亡，这很可能与跗骨感觉器官的形成有关。胚芽带分割似乎不需要细胞死亡。最重要的是，我们发现细胞死亡在胚芽带反转中起作用，这是蜘蛛特有的形态发生事件，涉及腹侧上皮分裂和背侧组织融合。我们的数据表明，胚带倒置涉及细胞死亡，以促进胚带的腹侧分裂，以及在背侧闭合期间上皮融合。科学通报，2005,(4):1 - 4。©2005 Wiley‐Liss, Inc。
Cell death is an important feature in the development of animals, because it is one possibility for the embryo to control cell number in developing tissues and thereby determine the form of a growing organ. In Drosophila, cell death has been shown to be involved in the shaping of many different organs of the embryo. We have used terminal deoxynucleotidyl transferase‐mediated dUTP‐digoxygenin nick end labeling (TUNEL) and an antibody against the activated form of caspase‐3 to visualize cell death in the spider Cupiennius salei. We find that similar to Drosophila, massive cell death occurs during the development of the nervous system, suggesting that this is an ancestral feature in the arthropods. We also detect cell death during leg development, most probably related to the formation of tarsal sensory organs. No cell death seems to be required for germ band segmentation. Most importantly, we find that cell death has a role in germ band inversion, a morphogenetic event unique to spiders that involves epithelial fission ventrally and tissue fusion dorsally. Our data show that germ band inversion involves cell death to facilitate the ventral splitting of the germ band, as well as the epithelial fusion during dorsal closure. Developmental Dynamics 234:222–228, 2005. © 2005 Wiley‐Liss, Inc.
影响因子：
2.7
作者：
Hua Shen;Todd A. Wilke;A. Ashique;M. Narvey;T. Zerucha;E. Savino;T. Williams;J. Richman
通讯作者：
Hua Shen;Todd A. Wilke;A. Ashique;M. Narvey;T. Zerucha;E. Savino;T. Williams;J. Richman
果蝇胚胎发生过程中的程序性细胞死亡。
DOI：
10.1242/dev.117.1.29
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Abrams,JM;White,K;Fessler,LI;Steller,H
通讯作者：
Steller,H