Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine
Specific probiotic characterization of Weissella hellenica DS-12 isolated from flounder intestine
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DOI：
10.2323/jgam.44.311
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发表时间：
1998-10-01
影响因子：
1.2
通讯作者：
Oh, TK
中科院分区：
文献类型：
--
作者：
Cai, YM;Benno, Y;Oh, TK
在韩国首尔的一个养鱼场，从比目鱼 (Paralichthys olivaceus) 的肠道内容物中分离出总共 199 种微生物。在这些菌株中，DS-12 被选为牙鲆益生菌的候选菌株，因为它对爱德华氏菌、巴氏杆菌、气单胞菌和弧菌等鱼类病原体具有出色的抗菌活性，并在 10% 氯化钠、10% 胆汁和 pH 3 的肉汤中启动生长 90 分钟。该菌株为革兰氏阳性、过氧化氢酶阴性的球状杆状体，由葡萄糖产生气体，并形成 90% 以上的 D(-) 异构体乳酸。根据 16S rRNA 序列，该生物体位于魏斯氏菌属中的一个簇，基于 16S rRNA 序列，根据 DNA-DNA 相关性将其分配给希腊魏斯氏菌。然而，W, hellenica JCM 10103(T)模式菌株对鱼类病原菌没有抗菌活性，并且在α-甲基-D-葡萄糖苷、七叶碱、纤维二糖、蜜二糖、D-棉子糖和D-松二糖的一些糖发酵模式上与DS-12菌株有很大不同，特别是在15和35℃的7% NaCl和10%胆汁，本研究结果表明W, hellenica菌株不具有益生菌特性，但菌株DS-12可以作为比目鱼的特异性益生菌。
A total of 199 microorganisms were isolated from the intestinal contents of flounder (Paralichthys olivaceus) in a fish farm in Seoul, Korea. Among these strains, DS-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% NaCl, 10% bile, and in broth at pH 3 for 90 min. This strain was Gram-positive, and catalase-negative coccoid rods that produced gas from glucose and formed more than 90% of lactate as the D(-) isomer, This organism is positioned at a cluster in the genus Weissella on the phylogenetic tree based on 16S rRNA sequences, which were assigned to Weissella hellenica on the basis of DNA-DNA relatedness. However, the type strain of W, hellenica JCM 10103(T) had no antibacterial activity against the fish pathogenic bacteria and was found to be quite different from the DS-12 strain in some sugar fermentation patterns of alpha-methyl-D-glucoside, esculine, cellobiose, melibiose, D-raffinose, and D-turanose, being especially unable to grow at 15 and 35 degrees C in 7% NaCl and 10% bile, The results obtained in the present study demonstrated that the type strain of W, hellenica had no probiotic characteristics, but the strain DS-12 could be used as a specific probiotic for flounder.