A conditional mutant having paralyzed cilia and a block in cytokinesis is rescued by cytoplasmic exchange in Tetrahymena thermophila.
A conditional mutant having paralyzed cilia and a block in cytokinesis is rescued by cytoplasmic exchange in Tetrahymena thermophila.
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嗜热四膜虫中纤毛麻痹和胞质分裂受阻的条件突变体通过细胞质交换得以挽救。
DOI：
10.1093/genetics/120.3.697
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Gorovsky,MA
中科院分区：
文献类型：
--
作者：
Pennock,DG;Thatcher,T;Bowen,J;Bruns,PJ;Gorovsky,MA
分离了十九个突变体，它们对于去纤毛后恢复运动能力和生长能力都是有条件的。导致生长缓慢的突变被分为五个互补组。所有突变似乎都不会影响能量产生，因为所有突变体在限制温度下均保持活动状态。在三个互补组中，蛋白质合成和编码α-微管蛋白或肌动蛋白的mRNA水平在限制温度下基本上不受影响，这与这三个组中的突变直接影响功能性纤毛的组装和生长的假设一致。选择互补组 1 进行进一步表征。两种表型都被证明是相关的，表明它们是由单一突变引起的。第 1 组突变体在限制温度下再生了纤毛，但纤毛不活动。这种突变还在限制温度下导致胞质分裂受阻，但不影响核分裂或 DNA 合成。当突变体在接合过程中与野生型细胞配对（基因组排除的第一轮）时，细胞分裂的阻碍被交换的野生型细胞质暂时挽救。因此，至少分离出一种突变，该突变影响四膜虫（再生期间的纤毛）中某些基于微管的结构的组装，但不影响其他结构（细胞核以 38 度分裂），并且该基因的产物可能在纤毛功能和胞质分裂中发挥作用。
Nineteen mutants that are conditional for both the ability to regain motility following deciliation and the ability to grow were isolated. The mutations causing slow growth were placed into five complementation groups. None of the mutations appears to affect energy production as all mutants remained motile at the restrictive temperature. In three complementation groups protein synthesis and the levels of mRNA encoding alpha-tubulin or actin were largely unaffected at the restrictive temperature, consistent with the hypothesis that mutations in these three groups directly affect the assembly of functional cilia and growth. Complementation group 1 was chosen for further characterization. Both phenotypes were shown to be linked, suggesting they are caused by a single mutation. Group 1 mutants regenerated cilia at the restrictive temperature, but the cilia were nonmotile. This mutation also caused a block in cytokinesis at the restrictive temperature but did not affect nuclear divisions or DNA synthesis. The block in cell division was transiently rescued by wild-type cytoplasm exchanged when mutants were paired with wild-type cells during conjugation (round 1 of genomic exclusion). Thus, at least one mutation has been isolated that affects assembly of some microtubule-based structures in Tetrahymena (cilia during regeneration) but not others (nuclei divide at 38 degrees), and the product of this gene is likely to play a role in both ciliary function and in cytokinesis.
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维拉帕米竞争性抑制大鼠心肌中的α1-肾上腺素能受体和毒蕈碱受体，但不抑制β-肾上腺素能受体。
影响因子：
3
作者：
Karliner,JS;Motulsky,HJ;Dunlap,J;Brown,JH;Insel,PA
通讯作者：
Insel,PA
揭示阿拉甲辛对心脏肌膜囊泡的 (Na ,K )-ATP 酶、β-肾上腺素受体偶联腺苷酸环化酶和 cAMP 依赖性蛋白激酶活性的影响。
影响因子：
4.8
作者：
L. Jones;S. Maddock;H. R. Besch
通讯作者：
H. R. Besch
心脏细胞膜的毒蕈碱受体与激动剂、拮抗剂和抗心律失常药的关联
影响因子：
3.5
作者：
D. Cavey;Jean;Michel Lazdunski
通讯作者：
Michel Lazdunski
奎尼丁是 α1 和 α2 肾上腺素受体的竞争性拮抗剂。
影响因子：
20.1
作者：
Motulsky,HJ;Maisel,AS;Snavely,MD;Insel,PA
通讯作者：
Insel,PA
维拉帕米对大鼠心脏毒蕈碱受体结合特性的影响：变构位点的证据。
DOI：
10.1016/0006-291x(84)90728-9
发表时间：
1984
影响因子：
3.1
作者：
M. Waelbroeck;P. Robberecht;P. Neef;Jean Christophe
通讯作者：
Jean Christophe