Brain ethanol levels in rats after voluntary ethanol consumption using a sweetened gelatin vehicle
Brain ethanol levels in rats after voluntary ethanol consumption using a sweetened gelatin vehicle
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DOI：
10.1016/j.pbb.2006.10.010
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发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Rowland, N. E.
中科院分区：
文献类型：
--
作者：
Peris, J.;Zharikova, A.;Rowland, N. E.
从启动的简易性、一致性和由此产生的大脑酒精水平方面对一种新的启动大鼠自愿酒精消耗的程序进行了评估。“果冻”由明胶中10%的乙醇和热量来源(多聚糖)组成。在Spraogue-Dawley大鼠中开始“果冻”摄入不需要限制食物或水，导致最初每天(8.4+/-0.6克/千克体重)和最终每小时(11+/-0.1克/千克/千克体重)的酒精摄入量与使用酒精偏好或非基因选择的大鼠的其他程序相当。通过“果冻注射”摄入乙醇的大鼠在环境改变和外科手术植入导尿管后很快恢复。在1小时的自由接触过程中，在最初的10分钟内，“果冻”的消耗发生了，并导致用微透析法测量的伏隔核内乙醇水平的剂量相关增加。这些大脑酒精水平与使用其他自我给药方法获得的水平相当。然而，当灌胃0.5g/kg乙醇时，无论是在“果冻”中还是在生理盐水中，灌胃“果冻”后，大脑中的乙醇浓度与生理盐水相比下降了约20%。即便如此，不需要最初的食物或水的匮乏，以及能够迅速实现稳定的自我管理，这两个因素都表明，作为一种完全自愿的乙醇过程，可以使用“果冻注射”。(C)2006 Elsevier Inc.保留所有权利。
A novel procedure for initiation of voluntary ethanol consumption in the rat was evaluated in terms of ease of initiation, consistency, and resulting brain ethanol levels. The "jello shot" consists of 10% ethanol in gelatin along with a caloric source (Polycose). Initiation of "jello shot" consumption in Sprague-Dawley rats required no food or water restriction and resulted in initial daily (8.4 +/- 0.6 g/kg body weight) and eventual hourly (11 +/- 0.1 g/kg body weight) intake of ethanol comparable to other procedures using either alcohol-preferring or non-genetically selected rats. Rat intake of ethanol via "jello shots" recovered quickly from environmental alterations and surgical implantation of a guide cannula. During 1-h free access sessions, consumption of the "jello shot" occurred during the initial 10 min and resulted in a dose-related increase in ethanol levels in nucleus accumbens measured using microdialysis. These brain ethanol levels were comparable to those achieved using other self-administration methods. However, when 0.5 g/kg ethanol was gavaged either in "jello shot" or saline, there was about a 20% decrease in brain ethanol concentrations after gavage of the "jello shot" compared to saline. Even so, lack of a need for initial food or water deprivation and the rapidity with which stable self-administration can be achieved both suggest utility of the "jello shot" as a completely voluntary ethanol procedure. (c) 2006 Elsevier Inc. All rights reserved.