Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria lemaneiformis Bory
Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria lemaneiformis Bory
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龙须菜不同世代枝四孢子释放条件优化及光合活性评价
DOI：
10.1007/s00343-010-9908-2
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发表时间：
2010
影响因子：
--
通讯作者：
Peng Guang
中科院分区：
文献类型：
--
作者：
Wang Zhiyuan;Wang Guangce;Niu Jianfeng;Wang Wenjun;Peng Guang
江蓠是一种经济上重要的藻类，主要用于琼脂生产。虽然四孢子是羊草的理想种子，但目前最流行的培养方法是基于营养片段的培养方法，这是一种劳动密集型和耗时的方法。在本研究中，我们优化了四孢子释放的条件，并利用PAM（脉冲振幅调制）测量系统对羊草分枝形成的不同菌落的光合活性进行了评价。结果表明，温度和盐度的变化对四孢子的产量有显著影响。然而，光子通量密度的变化（从15 μmol m−2 s−1到480 μmol m−2 s−1）对四孢子产量没有明显影响。不同分支形成的菌落的pam -参数Y(I)、Y（II）、ETR(I)、ETR（II）和Fv/Fm的变化趋势相同：第一代分支>；第二代分支>；第三代分支的参数值。这些结果表明，不同菌落枝条的光合活性具有相同的变化趋势。此外，研究还发现，lemaneiformis的光合作用参与了营养繁殖和四孢子的形成。最后，第一代枝条生长缓慢，但积累有机物形成大量的四孢子。综上所述，这些结果表明，第一代枝是释放四孢子的理想材料。
Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production. Although tetraspores are ideal seeds for the cultivation of G. lemaneiformis, the most popular culture method is currently based on vegetative fragments, which is labor-intensive and time-consuming. In this study, we optimized the conditions for tetraspore release and evaluated the photosynthetic activities of different colonies formed from the branches of G. lemaneiformis using a PAM (pulse-amplitude-modulated) measuring system. The results showed that variations in temperature and salinityhad significant effects on tetraspore yield. However, variations in the photon flux density (from 15 μmol m−2 s−1 to 480 μmol m−2 s−1) had no apparent effect on tetraspore yield. Moreover, the PAM-parameters Y(I), Y(II), ETR(I), ETR(II) and Fv/Fm of colonies formed from different branches showed the same trend: parameter values of first generation branches>second generation branches>third generation branches. These results suggest that the photosynthetic activities of different colonies of branches changed with the same trend. Furthermore, photosynthesis in G. lemaneiformis was found to be involved in vegetative reproduction and tetraspore formation. Finally, the first generation branches grew slowly, but accumulated organic compounds to form large numbers of tetraspores. Taken together, these results showed that the first generation branches are ideal materials for the release of tetraspores.