PROSTAGLANDIN ENDOPEROXIDES .7. NOVEL TRANSFORMATIONS OF ARACHIDONIC-ACID IN GUINEA-PIG LUNG
PROSTAGLANDIN ENDOPEROXIDES .7. NOVEL TRANSFORMATIONS OF ARACHIDONIC-ACID IN GUINEA-PIG LUNG
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DOI：
10.1016/0006-291x(74)90246-0
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
SAMUELSSON, B
中科院分区：
文献类型：
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作者：
HAMBERG, M;SAMUELSSON, B
[1-14C]花生四烯酸与豚鼠肺匀浆孵育后，分离并鉴定了下列标记化合物：12-羟基-5，8，10-十七碳三烯酸(HHT)，8-(1-hydroxy-3-oxopropyl)-9，12-dihydroxy-5，10-heptadecadienoic酸的半缩醛衍生物(PHD)，12-羟基-5，8，10，14-二十碳四烯酸(HETE)，前列腺素E_2，前列腺素F_2α，11-羟基-5，8，12，14-二十碳四烯酸和15-羟基-5，8，11，13-二十碳四烯酸(按产率递减)。灌流豚鼠肺注入30αg花生四烯酸后，释放PHD(65 4~2 30 4 ng)、HHT192~387 ng、HETE(66~111 ng)、PGE 2(15~93 ng)和PGF 2μ(93~171 ng)。因此，豚鼠肺匀浆和完整的豚鼠肺将添加的花生四烯酸主要转化为PHD和HHT，PHD和HHT是前列腺素内氧化PgG_2的代谢产物，少量转化为经典的前列腺素PGE_2和PGF_2α。
The following labeled compounds were isolated and identified after incubation of [1-14C]arachidonic acid with guinea pig lung homogenates: 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), the hemiacetal derivative of 8-(1-hydroxy-3-oxopropyl)-9,12-dihydroxy-5,10-heptadecadienoic acid (PHD), 12-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE), PGE2, PGF2α, 11-hydroxy-5,8,12,14-eicosatetraenoic acid, and 15-hydroxy-5,8,11,13-eicosatetraenoic acid (in order of decreasing yield). Perfused guinea pig lungs released PHD (654–2304 ng), HHT (192–387 ng), HETE (66–111 ng), PGE2(15–93 ng), and PGF2α(93–171 ng) following injection of 30 μg of arachidonic acid. Thus guinea pig lung homogenates as well as intact guinea pig lung converted added arachidonic acid predominantly into PHD and HHT, metabolites of the prostaglandin endoperoxide PGG2, and to a lesser extent into the classical prostaglandins PGE2and PGF2α.