A subpopulation of mushroom body intrinsic neurons is generated by protocerebral neuroblasts in the tobacco hornworm moth, Manduca sexta (Sphingidae, Lepidoptera)
A subpopulation of mushroom body intrinsic neurons is generated by protocerebral neuroblasts in the tobacco hornworm moth, Manduca sexta (Sphingidae, Lepidoptera)
复制标题
DOI：
10.1016/j.asd.2010.10.004
复制
发表时间：
2011-09-01
影响因子：
2
通讯作者：
Daly, Kevin C.
中科院分区：
文献类型：
--
作者：
Farris, Sarah M.;Pettrey, Colleen;Daly, Kevin C.
Kenyon细胞亚群是昆虫蘑菇体的内在神经元，通常由胚胎发生期间开始增殖的专用成神经细胞连续产生。当存在时，III类凯尼恩细胞被认为是第一个出生的神经元群体，因为它们的细胞胞体的位置离蘑菇体神经母细胞的位置最远。在烟草天蛾成虫中，III类凯尼恩细胞的轴突形成一个单独的Y道和背侧和腹侧小裂片;令人惊讶的是，这些独特的结构是不存在的幼虫天蛾蘑菇体。BrdU标记和免疫组织化学染色显示，III类凯尼恩细胞实际上是出生在中期幼虫通过成人阶段。它们的细胞体的外周位置是由于它们起源于两种以前未描述的原脑神经母细胞，与产生其他凯尼恩细胞类型的蘑菇体神经母细胞不同。这些发现挑战了所有Kenyon细胞仅由蘑菇体神经母细胞产生的概念，并可以解释为什么在昆虫中零星发现III类Kenyon细胞，这表明当存在时，它们可能是通过蘑菇体外神经母细胞的重新招募而产生的。此外，终身神经发生的III类神经母细胞和蘑菇体神经母细胞被观察到，提高成人神经发生可能发挥作用的蘑菇体功能在曼陀罗的可能性。(C)2010爱思唯尔有限公司保留所有权利。
Subpopulations of Kenyon cells, the intrinsic neurons of the insect mushroom bodies, are typically sequentially generated by dedicated neuroblasts that begin proliferating during embryogenesis. When present, Class III Kenyon cells are thought to be the first born population of neurons by virtue of the location of their cell somata, farthest from the position of the mushroom body neuroblasts. In the adult tobacco hornworm moth Manduca sexta, the axons of Class III Kenyon cells form a separate Y tract and dorsal and ventral lobelet; surprisingly, these distinctive structures are absent from the larval Manduca mushroom bodies. BrdU labeling and immunohistochemical staining reveal that Class III Kenyon cells are in fact born in the mid-larval through adult stages. The peripheral position of their cell bodies is due to their genesis from two previously undescribed protocerebral neuroblasts distinct from the mushroom body neuroblasts that generate the other Kenyon cell types. These findings challenge the notion that all Kenyon cells are produced solely by the mushroom body neuroblasts, and may explain why class III Kenyon cells are found sporadically across the insects, suggesting that when present, they may arise through de novo recruitment of neuroblasts outside of the mushroom bodies. In addition, lifelong neurogenesis by both the Class III neuroblasts and the mushroom body neuroblasts was observed, raising the possibility that adult neurogenesis may play a role in mushroom body function in Manduca. (C) 2010 Elsevier Ltd. All rights reserved.