Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors.
Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors.
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DOI：
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发表时间：
1985
影响因子：
6.5
通讯作者：
D. Figlewicz;C. Nolan;I. Singh;F. B. Jungalwala
中科院分区：
文献类型：
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作者：
D. Figlewicz;C. Nolan;I. Singh;F. B. Jungalwala
预填充反相色谱柱（Bond Elut）用于分离体外生物合成后的复合脂质，如磷脂酰胆碱、脑苷类、硫脂类和神经节苷类，从其各自的水溶性放射性前体中分离。在体外孵育后，整个反应混合物通过色谱柱，其中复杂的脂质被保留，亲水性放射性前体被从色谱柱中冲走。然后用更非极性的有机溶剂洗脱保留的脂质。这个过程比通常使用的Folch划分方法或其他过程更简单，更有效。
Pre-packed reverse phase columns (Bond Elut) were used for the separation of complex lipids, such as phosphatidylcholine, cerebrosides, sulfatides, and gangliosides, from their respective water-soluble radioactive precursors after their in vitro biosynthesis. After an incubation in vitro, the entire reaction mixture is passed through the column, where complex lipids are retained and the hydrophilic radioactive precursors are washed away from the column. The retained lipids are then eluted with a more nonpolar organic solvent. The procedure is shown to be simpler and more efficient than the normally used Folch partitioning method or other procedures.