Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum
Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum
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豌豆 (Pisum sativum) 被元宝炭疽菌 (Colletotrichum truncatum) 半生物营养感染
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
J. A. Bailey
中科院分区：
文献类型：
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作者：
R. O’Connell;A. Uronu;G. Waksman;C. Nash;J. Keon;J. A. Bailey
采用光镜和电镜对豌豆炭疽病菌（Colletotrichum truncatum）侵染进行了研究。这些研究是利用一个阿根廷豌豆突变体，它有一个容易分离的表皮。附着胞产生的感染钉直接穿透表皮细胞。大的细胞内初级菌丝形成致密的间质菌丝，被限制在单个表皮细胞内。初级菌丝产生较薄的次级菌丝，次级菌丝向周围细胞辐射，引起广泛的细胞壁溶解。褪绿水浸泡的病灶中心出现黑化的巩膜。未见针状体。表皮细胞在初代菌丝渗透时存活，表现为其酶解能力和中性红色的积累，但在次生菌丝形成时，所有被感染的细胞都死亡。6个豌豆品种易感，但其他7个豆科植物具有抗性。从培养滤液中纯化出pI为8·3，表观Mr为40000的聚半乳糖醛酸酶单异构体，并测定了tv端氨基酸序列。本文还讨论了研究结果与柽柳分类学的相关性以及侵染过程与寄主范围的关系。
The infection of pea (Pisum sativum) by Colletotrichum truncatum was studied by light and electron microscopy. These investigations were facilitated by use of an Argenteum pea mutant, which has a readily detachable epidermis. Infection pegs emerging from appressoria penetrated epidermal cells directly. Large intracellular primary hyphae formed a dense stromatic mycelium confined within a single epidermal cell. Primary mycelia gave rise to thinner secondary hyphae which radiated into surrounding cells and caused extensive wall dissolution. Melanized sclerotia developed in the centre of chlorotic water-soaked lesions. Acervuli were not observed. Epidermal cells survived initial penetration by primary hyphae, as shown by their ability to plasmolyse and accumulate Neutral red, but all infected cells were dead when the secondary hyphae had formed. Six cultivars of pea were susceptible, but seven other legumes were resistant. A single isoform of polygalacturonase with a pI of 8·3 and apparent Mr of 40000 was purified from culture filtrates and the TV-terminal amino acid sequence determined. The relevance of the results to the taxonomy of C. truncatum and the relationships between infection process and host range are discussed.