Black Soybean Seed Coat Extract Ameliorates Hyperglycemia and Insulin Sensitivity via the Activation of AMP-Activated Protein Kinase in Diabetic Mice
Black Soybean Seed Coat Extract Ameliorates Hyperglycemia and Insulin Sensitivity via the Activation of AMP-Activated Protein Kinase in Diabetic Mice
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DOI：
10.1021/jf401190y
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发表时间：
2013-06-12
影响因子：
6.1
通讯作者：
Tsuda, Takanori
中科院分区：
文献类型：
--
作者：
Kurimoto, Yuta;Shibayama, Yuki;Tsuda, Takanori
黑豆种皮含有丰富的多酚类物质，如花青素（矢车菊素3-葡萄糖苷; C3 G）和原花青素（PC）。本研究发现，饮食黑豆种皮提取物（BE）改善高血糖和胰岛素敏感性，通过激活AMP激活的蛋白激酶。（AMPK）在2型糖尿病小鼠中的作用。饮食BE显着降低血糖水平和增强胰岛素敏感性。AMPK在喂食BE的糖尿病小鼠的骨骼肌和肝脏中被激活。这种激活伴随着骨骼肌中葡萄糖转运蛋白4的上调和肝脏中糖异生的下调。这些变化导致2型糖尿病小鼠的高血糖和胰岛素敏感性改善。在体外研究中，使用L 6肌管表明，C3 G和PC显着诱导AMPK激活和增强葡萄糖摄取到细胞。
Black soybean seed coat has abundant levels of polyphenols such as anthocyanins (cyanidin 3-glucoside; C3G) and procyanidins (PCs). This study found that dietary black soybean seed coat extract (BE) ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase. (AMPK) in type 2 diabetic mice. Dietary BE significantly reduced blood glucose levels and enhanced insulin sensitivity. AMPK was activated in the skeletal muscle and liver of diabetic mice fed BE. This activation was accompanied by the up-regulation of glucose transporter 4 in skeletal muscle and the down-regulation of gluconeogenesis in the liver. These changes resulted in improved hyperglycemia and insulin sensitivity in type 2 diabetic mice. In vitro studies using L6 myotubes showed that C3G and PCs significantly induced AMPK activation and enhanced glucose uptake into the cells.