Functional heterogeneity of side population cells in skeletal muscle
Functional heterogeneity of side population cells in skeletal muscle
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DOI：
10.1016/j.bbrc.2006.01.037
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发表时间：
2006-03-17
影响因子：
3.1
通讯作者：
Takeda, S
中科院分区：
文献类型：
--
作者：
Uezumi, A;Ojima, K;Takeda, S
骨骼肌的再生完全归因于肌源性前体细胞，卫星细胞。富含干细胞的部分称为侧群（SP）细胞也存在于骨骼肌中，但其在肌肉再生中的作用仍不清楚。我们发现，肌肉SP细胞可以细分为三个亚组使用CD 31和CD 45标记。在正常非再生肌肉中，大多数SP细胞表达CD 31，具有内皮特征。然而，CD 31（-）CD 45（-）SP细胞，这是一个小的亚群在正常的肌肉，积极增殖的肌肉损伤后，不仅表达几个调控基因的肌肉再生，但也有一些间充质谱系标记。CD 31（-）CD 45（-）SP细胞在三个SP亚群中显示出最大的肌源性潜能，但在体外确实显示出间充质潜能。这些SP细胞在体内肌内移植后优先分化为肌纤维。我们的研究结果揭示了肌肉SP细胞的异质性，并表明CD 31（-）CD 45（-）SP细胞参与肌肉再生。(c)2006爱思唯尔公司All rights reserved.
Skeletal muscle regeneration has been exclusively attributed to myogenic precursors, satellite cells. A stem cell-rich fraction referred to as side population (SP) cells also resides in skeletal muscle, but its roles in muscle regeneration remain unclear. We found that muscle SP cells could be subdivided into three sub-fractions using CD31 and CD45 markers. The majority of SP cells in normal non-regenerating muscle expressed CD31 and had endothelial characteristics. However, CD31(-)CD45(-)SP cells, which are a minor subpopulation in normal muscle, actively proliferated upon muscle injury and expressed not only several regulatory genes for muscle regeneration but also some mesenchymal lineage markers. CD31(-)CD45(-) SP cells showed the greatest myogenic potential among three SP sub-fractions, but indeed revealed mesenchymal potentials in vitro. These SP cells preferentially differentiated into myofibers after intramuscular transplantation in vivo. Our results revealed the heterogeneity of muscle SP cells and suggest that CD31(-)CD45(-) SP cells participate in muscle regeneration. (c) 2006 Elsevier Inc. All rights reserved.