An immune-responsive serpin regulates the melanization cascade in Drosophila
An immune-responsive serpin regulates the melanization cascade in Drosophila
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DOI：
10.1016/s1534-5807(02)00267-8
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发表时间：
2002-10-01
影响因子：
11.8
通讯作者：
Brey, PT
中科院分区：
文献类型：
--
作者：
De Gregorio, E;Han, SJ;Brey, PT
在节肢动物中，黑化反应与多种宿主防御机制相关，导致入侵微生物的隔离和杀死。节肢动物的黑化是由丝氨酸蛋白酶的级联反应控制的，丝氨酸蛋白酶最终激活了酚氧化酶原（PPO），进而催化黑色素的合成。在这里，我们报告的生化和遗传特性的果蝇丝氨酸蛋白酶抑制剂蛋白，丝氨酸蛋白酶抑制剂-27A，它通过特异性抑制末端蛋白酶酚氧化酶原激活酶调节黑化级联反应。我们的数据表明，Serpin-27 A需要限制酚氧化酶活性的损伤或感染的网站，防止昆虫过度黑化。
In arthropods, the melanization reactions is associated with multiple host defense mechanisms leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.