A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5αs
A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5αs
复制标题
人类免疫缺陷病毒 2 型衣壳的单个氨基酸在食蟹猴和人类 TRIM5α 存在的情况下影响其复制
作者：
Haihan Song;E. Nakayama;M. Yokoyama;Hironori Sato;J. Levy;T. Shioda
人类免疫缺陷病毒2型（HIV-2）毒株在旧大陆猴（OWM）细胞如食蟹猴（CM）细胞中生长的能力差异很大。我们评估了8个HIV-2分离株对CM TRIM 5 α（OWM细胞中的抗HIV因子）的敏感性。我们发现不同的HIV-2分离株对CM TRIM 5 α的敏感性存在差异。序列分析表明，TRIM 5 α敏感病毒在衣壳蛋白（CA）的第120位有脯氨酸，而TRIM 5 α抗性病毒则有丙氨酸或谷氨酰胺。突变研究表明，第120位的单个氨基酸确实影响了病毒对CM TRIM 5 α的敏感性。
ABSTRACT Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their abilities to grow in Old World monkey (OWM) cells such as those of cynomolgus monkeys (CM). We evaluated eight HIV-2 isolates for their sensitivities to CM TRIM5α, an anti-HIV factor in OWM cells. We found that different HIV-2 isolates showed differences in their sensitivities to CM TRIM5α. Sequence analysis showed that TRIM5α-sensitive viruses had proline at the 120th position of the capsid protein (CA), whereas TRIM5α-resistant viruses had either alanine or glutamine. Mutagenesis studies indicated that the single amino acid at the 120th position indeed affected the sensitivity of the virus to CM TRIM5α.
DOI：
10.1073/pnas.0403364101
发表时间：
2004-08-10
影响因子：
11.1
作者：
Perron, MJ;Stremlau, M;Sodroski, J
通讯作者：
Sodroski, J
DOI：
10.1073/pnas.0409853102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Sawyer, SL;Wu, LI;Malik, HS
通讯作者：
Malik, HS
DOI：
10.1073/pnas.0402361101
发表时间：
2004-07-20
影响因子：
11.1
作者：
Hatziioannou, T;Perez-Caballero, D;Bieniasz, PD
通讯作者：
Bieniasz, PD