RNA-Seq analysis reveals genetic bases of the flowering process in oriental hybrid lily cv. Sorbonne
RNA-Seq analysis reveals genetic bases of the flowering process in oriental hybrid lily cv. Sorbonne
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RNA-Seq 分析揭示了东方杂交百合品种开花过程的遗传基础。
DOI：
10.1134/s1021443714060132
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发表时间：
2014-11-01
影响因子：
1.4
通讯作者：
Lu, Y. M.
中科院分区：
文献类型：
--
作者：
Liu, X. H.;Huang, J.;Lu, Y. M.
开花是植物繁殖的关键步骤。东方百合(Lilium)花色艳丽，用途日益广泛，但花期是影响百合价值的重要限制因素。利用分子育种技术改良百合开花具有广阔的应用前景，但百合开花调控机制尚待研究。根据RNA-Seq数据组装了东方百合开花过程中转录变异的全面序列图谱数据。将大约1.2416亿个90bp成对的末端清洁读数组装成66个327个非基因片段，并与UniProt数据库进行比较。与UniProt数据库中的序列有显著匹配的非基因有30 254个，分别有60 738个、16 601个和12 494个非基因与GO、KEGG和COG数据库相似。通过对百合开花过程中转录组的动态分析，发现了一些与百合开花相关的基因，揭示了百合开花过程中在转录组水平上复杂的开花调控网络。此外，实时荧光定量RT-PCR分析还鉴定了与开花相关的12个基因，包括LoLFY、LoMAF、LOFT、LoAG、LoCBF、LoAGL6a、LoSOC1、LoSEP1、LoNAC1、LoAPX、LoARF10和LoICE。结果表明，东方百合的花在不同的开花阶段都具有较高比例的开花基因。根据本研究的结果，我们预测它们将在东方百合的开花过程中发挥重要作用，这些数据为进一步研究东方百合开花过程中的代谢提供了基础。
Flowering is a critical step in plant reproduction. Oriental lily (Lilium) characterized by showy flowers is increasingly used; however, the blossom period is an important limiting factor influencing the value of lily. Improving the flowering of lily by molecular breeding technology has limitless applications, but the mechanism of the regulation of lily flowering remains to be studied. Transcriptomes providing comprehensive sequence profiling data of transcription variation during flowering process in Oriental hybrids lily Sorbonne were assembled from RNA-Seq data. Approximately 124.16 million 90 bp paired-end clean reads were assembled into 66 327 unigenes and compared with the UniProt databases. There are 30 254 unigenes that have significant hits to the sequences in the UniProt database, 60 738, 16 601, and 12 494 unigenes have similarity to the GO, KEGG, and COG databases, respectively. By analyzing dynamic changes in the transcriptome of lily flowering based on our RNA sequencing (RNA-Seq) data, some genes involved in floral induction were found, which revealed the complicated flower regulation network at the transcriptome level during lily flowering. Moreover, 12 DEGs related to flowering including LoLFY, LoMAF, LoFT, LoAG, LoCBF, LoAGL6a, LoSOC1, LoSEP1, LoNAC1, LoAPX, LoARF10, and LoICE were identified with real-time quantitative RT-PCR analysis. The results suggested that the flower of Oriental lily possessed a high proportion of flowering genes active at different stages of flowering. According to the results of the present study, we predicted that they would play an important role during flowering process; these data provided the foundation for future studies of metabolism during flowering of Oriental lily.