Construction and analysis of a Pseudomonas-Saccharomyces microbial consortium for mcl-PHA production from xylose and octanoate
Construction and analysis of a Pseudomonas-Saccharomyces microbial consortium for mcl-PHA production from xylose and octanoate
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DOI：
10.1002/cjce.24751
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发表时间：
2023-01-10
影响因子：
2.1
通讯作者：
Jia，Xiaoqiang
中科院分区：
文献类型：
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作者：
Wei，Zixiang;Zhu，Yinzhuang;Jia，Xiaoqiang
微生物菌群具有较强的综合生产能力，在生产聚羟基脂肪酸酯（PHAs）方面具有巨大的潜力。本研究以恶臭假单胞菌（PseudomonasputidaNBRC 14164）和嗜酸乳杆菌（SaccharomycesgallaeSyBE_Sc01020078）为菌种，构建了一个能将木糖转化为P. putidabyS.利用木糖和辛酸酯生产中链聚羟基脂肪酸酯（mcl-PHA），并初步分析了两株菌之间的相互作用。最佳接种比例为1%恶臭假单胞菌+10%硫代硫酸杆菌。酿酒酵母，并且两种菌株在其中期指数期表现出最高的PHA产量。这两种微生物在矿物盐培养基中生长，木糖和辛酸盐作为底物，不洗涤，并以200 rpm培养。该菌群的mcl-PHA滴度为295.7 mg/L，比P. putidain纯文化.通过气相色谱-质谱联用技术分析表明，P. putida和S.酿酒酵母，其中前者为后者提供组氨酸、甲硫氨酸和亮氨酸，而后者将木糖转化为乳酸，乳酸是前者更容易获得的碳源。
Microbial consortia have great potential for the production of polyhydroxyalkanoates (PHAs) due to their comprehensive capability. In this study, a microbial consortium consisting ofPseudomonas putidaNBRC14164 andSaccharomyces cerevisiaeSyBE_Sc01020078 was constructed where xylose was converted into nutrients utilizable toP. putidabyS. cerevisiaeto realize the production of medium‐chain‐length polyhydroxyalkanoates (mcl‐PHAs) from xylose and octanoate, followed by a preliminary analysis of the interaction between the two strains. The optimal inoculation ratio consisted of 1%P. putidaand 10%S. cerevisiae, and both strains exhibited the highest PHA production at their mid‐exponential phase. The two microorganisms were grown in a mineral salt medium with xylose and octanoate as substrates without washing and cultured at 200 rpm. The mcl‐PHA titre of this consortium was 295.7 mg/L, which was 150% higher than that ofP. putidain pure culture. Metabolites analysis by gas chromatography–mass spectrometry revealed metabolic communication betweenP. putidaandS. cerevisiae, whereby the former provided histidine, methionine, and leucine for the latter, while the latter converted xylose into lactic acid, which is a more accessible carbon source for the former.