Tendon Tissue Engineering Using Cell-Seeded Umbilical Veins Cultured in a Mechanical Stimulator
Tendon Tissue Engineering Using Cell-Seeded Umbilical Veins Cultured in a Mechanical Stimulator
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DOI：
10.1089/ten.tea.2008.0102
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发表时间：
2009-04-01
影响因子：
4.1
通讯作者：
Sikavitsas, Vassilios
中科院分区：
文献类型：
--
作者：
Abousleiman, Rita I.;Reyes, Yuliana;Sikavitsas, Vassilios
本研究的目的是研究周期性机械刺激对人脐静脉（HUVs）内接种的间充质干细胞（MSC）的影响，并确定工程结构作为肌腱组织替代模型的潜力。将脱细胞的HUV与包埋在I型胶原水凝胶中的MSC接种。专门设计了用于组织工程应用的机械刺激器，用于循环拉伸结构长达2周，其中对照组保持未拉伸。与仅由细胞胶原凝胶组成的其他肌腱组织工程构建体相比，该接种有细胞胶原凝胶的HUV模型系统加上机械刺激导致机械性能的改善，而没有任何额外的支撑支架。培养2周后，测量到张紧和未张紧结构的细胞数量增加;然而，刺激样品的增加至少高出8倍。显微镜下，周期性拉伸的样本显示平行取向的胶原纤维和梭形细胞核模仿天然肌腱的形态。此外，与未拉伸样本相比，机械刺激导致结构显著更强（156%）和更硬（109%）。这种工程化肌腱模型的极限拉伸强度值仅比人类肌腱低一个数量级，应变值在人类肌腱的范围内。记录的结果是有前途的，可以进一步改善，通过优化潜在的关键文化参数，如接种密度，加载制度，和mechanostimulation持续时间。
The goal of this study was to investigate the effect of cyclic mechanical stimulation on mesenchymal stem cells (MSCs) seeded within human umbilical veins (HUVs), and to determine the potential of the engineered constructs to function as tendon tissue replacement models. Decellularized HUVs were seeded with MSCs embedded in type I collagen hydrogel. A mechanical stimulator for tissue engineering applications was specifically designed to cyclically tension the constructs for durations up to 2 weeks, where controls were left untensioned. This HUV model system seeded with a cellular collagen gel, coupled with mechanical stimulation, resulted in improved mechanical properties compared to other tendon tissue engineered constructs composed of cellular collagen gel alone, without any additional supporting scaffold. After 2 weeks of culture an increase in cell number was measured for both tensioned and untensioned constructs; however, the increase was at least eightfold higher for stimulated samples. Microscopically, cyclically tensioned samples showed parallel orientation of collagen fibers and spindle-shaped cell nuclei mimicking the morphology of native tendons. Moreover, mechanostimulation resulted in significantly stronger (156%) and stiffer (109%) constructs compared to untensioned samples. This engineered tendon model had an ultimate tensile strength value only one order of magnitude lower than human tendons and strain values in the range of human tendons. The results documented are promising and can be further improved by optimizing potentially critical culture parameters such as seeding density, loading regimes, and mechanostimulation durations.