ALTERED MITOGENIC RESPONSIVENESS OF CHRONIC LEUKEMIC LYMPHOCYTES AND NORMAL HUMAN LYMPHOCYTES TREATED WITH DIMETHYL-SULFOXIDE
ALTERED MITOGENIC RESPONSIVENESS OF CHRONIC LEUKEMIC LYMPHOCYTES AND NORMAL HUMAN LYMPHOCYTES TREATED WITH DIMETHYL-SULFOXIDE
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DOI：
10.1111/j.1749-6632.1975.tb25345.x
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发表时间：
1975-01-01
影响因子：
5.2
通讯作者：
WILSON, HE
中科院分区：
文献类型：
--
作者：
DENNIS, AJ;WILSON, HE
与正常供体的淋巴细胞相比，慢性淋巴细胞白血病患者的外周淋巴细胞对 PHA 刺激的反应延迟且降低。该反应是通过测量氚化胸苷的掺入来确定的。在经PHA刺激的正常细胞培养物的培养液中添加浓度为0.1%、0.25%、0.5%、1.0%和2.0%(v/v)的DMSO，在3天的测试期间引起标记胸苷的掺入率增加3至4倍。事实证明，添加 2.0% DMSO 48 小时后优先对白血病细胞培养物产生细胞毒性，而对正常淋巴细胞培养物几乎没有细胞毒性。在用 PHA 刺激的白血病细胞培养物中添加 0.1%、0.25%、0.5% 和 1.0% DMSO（与正常细胞培养物一样），导致 3 天时氚化胸苷的摄取率增加 6 至 6.5 倍。
Peripheral lymphocytes from patients with chronic lymphocytic leukemia display a delayed and lowered response to stimulation by PHA, as compared with the lymphocytes from normal donors. This response was determined by measuring the incorporation of tritiated thymidine. The addition of DMSO in concentrations of 0.1%, 0.25%, 0.5%, 1.0%, and 2.0%(v/v) to the culture fluid of normal cell cultures that had been stimulated, with PHA elicited a 3-to 4-fold increase in the rate of incorporation of labeled thymidine at the 3-day test period. The addition of 2.0% DMSO proved to be preferentially cytotoxic to cultures of leukemic cells after 48 hours, as compared with the virtual absence of cytotoxicity for cultures of normal lymphocytes. The addition of 0.1%, 0.25%, 0.5%, and 1.0% DMSO to cultures of leukemic cells stimulated with PHA (as were normal cell cultures) resulted in a 6-to 6.5-fold increase in the rate of uptake of tritiated thymidine at 3 days.