Nickel induces oscillatory behavior and enhanced synaptic and electrotonic transmission between stomatogastric neurons of Panulirus interruptus.
Nickel induces oscillatory behavior and enhanced synaptic and electrotonic transmission between stomatogastric neurons of Panulirus interruptus.
复制标题
镍诱导振荡行为并增强中断龙虾口胃神经元之间的突触和电紧张传递。
DOI：
10.1016/0006-8993(93)91087-9
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Graubard,K
中科院分区：
文献类型：
--
作者：
Zirpel,L;Baldwin,D;Graubard,K
口胃神经节的幽门模式产生网络使用爆发诱发平台电位、突触传递和电偶联的混合来产生其模式输出。本研究考察了两种二价钙通道阻滞剂镍和镉对断尾蕨幽门扩张（PD）神经元和幽门外侧（LP）神经元之间电压振荡、突触传递和电偶联的影响。将体外口胃神经节浸泡在含河豚毒素（TTX）的生理盐水中，消除na -spike和幽门节律的自发电压振荡，使其静息电位稳定。在TTX生理盐水中加入50-100 μ M Ni2+，引起与内源性节律性活动相似的振幅和频率的电压振荡（应用TTX之前）。25-50 μM镍增强了突触的梯度传递和电耦合，改变了电压波形，而对胞体的输入电阻几乎没有影响。10-1000 μM Cd2+作为分级突触传递的剂量依赖性阻断剂，但没有其他可检测到的作用。我们认为，与镉不同，镍在幽门神经深部发挥一种类似调制器的作用。
The pyloric pattern generator network of the stomatogastric ganglion uses a mixture of burst-inducing plateau potentials, synaptic transmission, and electrical coupling to produce its patterned output. This study examines the effects of two divalent, calcium channel blockers, nickel and cadmium, on voltage oscillations, synaptic transmission, and electrical coupling between the two pyloric dilator (PD) neurons and lateral pyloric (LP) neuron ofPanulirus interruptus. The in vitro stomatogastric ganglion was bathed in saline containing tetrodotoxin (TTX) to eliminate Na-spikes and the spontaneous voltage oscillations of the pyloric rhythm, resulting in a steady resting potential. Addition of 50–100 μ M Ni2+to the TTX-saline induced voltage oscillations of similar amplitude and frequency as the endogenous rhythmic activity (before the application of TTX). 25–50 μM nickel enhanced graded synaptic transmission and electrical coupling and altered voltage waveforms, while producing little change in the input resistance measured in the soma. 10–1000 μM Cd2+acted as a dose-dependent blocker of graded synaptic transmission, but had no other detectable effects. We propose that nickel, in contrast to cadmium, exerts a modulator-like effect deep in the pyloric neuropil.