Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection
Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection
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DOI：
10.1016/j.vetpar.2005.10.016
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发表时间：
2006-01-15
影响因子：
2.6
通讯作者：
Singh, BP
中科院分区：
文献类型：
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作者：
Sriveny, D;Raina, OK;Singh, BP
从巨大片形吸虫中提取的组织蛋白酶L半胱氨酸蛋白酶被评估为其在牛的这种蠕虫感染的早期专利前检测的潜力。实验用400个囊蚴/头感染5头杂交犊牛，观察其抗组织蛋白酶L抗体反应。使用ELISA、试纸ELISA和Western blotting检测感染后4周的犊牛巨型赤体菌感染，灵敏度100%。用ELISA法对256份牛血清进行了检测，结果表明，总感染率为26.95%。初步研究表明，巨虫组织蛋白酶L半胱氨酸蛋白酶与表皮副胃、巨子叶外植体和包虫囊抗原无交叉反应。然而，在该抗原被认为适合于这些反刍动物的片形虫病的免疫诊断之前，需要对该抗原与牛和水牛的相关寄生虫的交叉反应性进行广泛的研究。(c) 2005 Elsevier B.V.版权所有
Cathepsin L cysteine proteinase from Fasciola gigantica was evaluated for its potential in the early prepatent detection of this helminth infection in bovine calves. Five cross-bred bovine calves were experimentally infected with 400 metacercariae/calf and evaluated for anti-cathepsin L antibody response. E gigantica infection in these calves could be detected 4 weeks post-infection using an ELISA, dipstick ELISA and Western blotting with 100% sensitivity. The antigen was also used to detect E gigantica field infection in cattle, by screening 256 sera of these animals by an ELISA, which demonstrated an overall infection rate of 26.95%. Preliminary studies showed that F gigantica cathepsin L cysteine proteinase does not cross-react with Paramphistomum epiclitum, Gigantocotyle explanatum and hydatid cyst antigens. However, extensive studies on the cross-reactivity of this antigen with related helminth parasites of cattle and buffaloes are required, before this antigen can be considered suitable for immuno-diagnosis of fasciolosis in these ruminants. (c) 2005 Elsevier B.V. All rights reserved.