Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism.
Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism.
复制标题
纯化的花椰菜芽质体的脂质合成和碳水化合物代谢的酶能力。
DOI：
10.1104/pp.79.2.458
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发表时间：
1985
期刊：
影响因子：
7.4
通讯作者：
Roland Douce
中科院分区：
文献类型：
--
作者：
E. Journet;Roland Douce
分离的花椰菜（Brassica oleracea）芽质体，通过 Percoll 密度梯度等密度离心纯化，被发现高度完整，几乎没有质体外污染，并且保留了参与脂肪酸、磷脂酸和单半乳糖基二酰基甘油合成的所有酶。纯化的质体拥有将磷酸丙糖转化为淀粉所需的所有酶，反之亦然，并且能够将甘油酸3-磷酸转化为丙酮酸以合成脂肪酸。它们还能够氧化磷酸己糖并通过氧化戊糖磷酸途径转化为磷酸丙糖。因此，花椰菜芽质体被证明是生化上非常灵活的细胞器。
Isolated cauliflower (Brassica oleracea) bud plastids, purified by isopycnic centrifugation in density gradients of Percoll, were found to be highly intact, to be practically devoid of extraplastidial contaminations, and to retain all the enzymes involved in fatty acid, phosphatidic acid, and monogalactosyldiacylglycerol synthesis. Purified plastids possess all the enzymes needed to convert triose phosphate to starch and vice versa, and are capable of conversion of glycerate 3-phosphate to pyruvate for fatty acid synthesis. They are also capable of oxidation of hexose phosphate and conversion to triose phosphate via the oxidative pentosephosphate pathway. Cauliflower bud plastids prove to be, therefore, biochemically very flexible organelles.