Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp nov., Campylobacter lari subsp concheus subsp nov and Campylobacter lari subsp lari subsp nov.
Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp nov., Campylobacter lari subsp concheus subsp nov and Campylobacter lari subsp lari subsp nov.
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DOI：
10.1099/ijs.0.000851-0
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Vandamme, Peter
中科院分区：
文献类型：
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作者：
Debruyne, Lies;On, Stephen L. W.;Vandamme, Peter
为明确从贝类和人体分离的类拉里弯曲杆菌的分类地位，进行了多相研究。通过荧光扩增片段长度多态性分析和全细胞蛋白电泳初步筛选了菌株集合内的多样性，揭示了两个不同于C. lari和其他弯曲杆菌属物种。通过表型分析、16 S rRNA和hsp 60基因序列分析证实了这些聚类的分歧。系统发育分析表明，C. lari、空肠弯曲菌、结肠弯曲菌和岛生弯曲菌是这两个类群最接近的系统发育邻居。DNA-DNA杂交显示，一个簇，包括10个菌株，代表一个新的弯曲杆菌属物种，其名称为弯曲杆菌pelorobacter sp. nov.，2314 BVA（T）（=LMG 23910（T）=CCUG 55787（T））作为模式菌株。第二个集群，包括6个菌株，代表了一个新的亚种内的物种C。拉里弯曲杆菌（Campylobacter lari subsp.）贝壳亚种nov.，模式菌株为2897 R（T）（=LMG 21009（T）CCUG 55786（T））。对C.拉里亚种concheus具有自动产生亚种弯曲杆菌lari亚种的作用。拉里亚种nov.（模式菌株LMG 8846（T）=NCTC 11352（T））。
A polyphasic study was undertaken to clarify the taxonomic position of Campylobacter lari-like strains isolated from shellfish and humans. The diversity within the strain collection was initially screened by means of fluorescent amplified fragment length polymorphism analysis and whole-cell protein electrophoresis, revealing the existence of two clusters distinct from C. lari and other Campylobacter species. The divergence of these clusters was confirmed by phenotypic analysis and by 16S rRNA and hsp60 gene sequence analysis. Phylogenetic analysis identified C. lari, Campylobacter jejuni, Campylobacter coli and Campylobacter insulaenigrae as the closest phylogenetic neighbours of both taxa. DNA-DNA hybridizations revealed that one cluster, comprising 10 strains, represented a novel Campylobacter species, for which the name Campylobacter peloridis sp. nov. is proposed, with 2314BVA(T) (=LMG 23910(T) =CCUG 55787(T)) as the type strain. The second cluster, comprising six strains, represents a novel subspecies within the species C. lari, for which the name Campylobacter lari subsp. concheus subsp. nov. is proposed, with 2897R(T) (=LMG 21009(T) CCUG 55786(T)) as the type strain. The description of C. lari subsp. concheus has the effect of automatically creating the subspecies Campylobacter lari subsp. lari subsp. nov. (type strain LMG 8846(T)=NCTC 11352(T)).