Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity
Analysis of recombinant monoclonal antibody isoforms by electrospray ionization mass spectrometry as a strategy for streamlining characterization of recombinant monoclonal antibody charge heterogeneity
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DOI：
10.1016/j.ab.2005.10.003
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Mhatre, R
中科院分区：
文献类型：
--
作者：
Lyubarskaya, Y;Houde, D;Mhatre, R
通过阳离子交换色谱分析的治疗性重组单克隆抗体显示出由大约10种同种异构体组成的异质谱。通过电喷雾四极杆飞行时间质谱（ESI-q-TOF-MS）、n端Edman测序、肽图谱和其他技术对这些峰进行分离和表征。酸性（较低的pI）峰被发现代表脱酰胺和唾液的物种。较高的pI峰含有N端和c端重链变体。比较丰富的同种异构体的生物活性具有可比性。提出了一种简化抗体电荷异质性表征的方法。(c) 2005爱思唯尔公司版权所有。
A therapeutic recombinant monoclonal antibody analyzed by cation-exchange chromatography exhibited a heterogeneous profile composed of approximately 10 isoforms. The peaks were isolated and characterized by electrospray quadrupole time-of-flight mass spectrometry (ESI-q-TOF-MS), N-terminal Edman sequencing, peptide mapping, and other techniques. Acidic (lower pI) peaks were found to represent deamidated and sialyated species. Higher pI peaks were found to contain N- and C-terminal heavy-chain variants. Biological activities of the more abundant isoforms were found to be comparable. An approach streamlining the characterization of antibody charge heterogeneity is proposed. (c) 2005 Elsevier Inc. All rights reserved.