A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein in Escherichia coli
A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein in Escherichia coli
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DOI：
10.1002/anie.201000465
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发表时间：
2010-01-01
影响因子：
16.6
通讯作者：
Liu, Wenshe R.
中科院分区：
文献类型：
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作者：
Wan, Wei;Huang, Ying;Liu, Wenshe R.
Two 的公司：使用野生型或进化的 PylRS-pylT UUA 对来抑制赭石突变，并使用进化的 MjTyrRSMjtRNA CUA Tyr 对来抑制琥珀突变，两种不同的非常规氨基酸 (NAA) 已被高效地同时整合到大肠杆菌中的一种蛋白质中（见图，NAA 1-4；GFP = 绿色荧光蛋白）。“化学方程式”
Two's company: Using a wild-type or evolved PylRS-pylT UUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNA CUA Tyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP= green-fluorescent protein)." Chemical equation presented"