Genomic profiling of carbohydrate metabolism in the ectomycorrhizal fungus Tuber melanosporum
Genomic profiling of carbohydrate metabolism in the ectomycorrhizal fungus Tuber melanosporum
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DOI：
10.1111/j.1469-8137.2010.03520.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Stocchi, V.
中科院分区：
文献类型：
--
作者：
Ceccaroli, P.;Buffalini, M.;Stocchi, V.
在外生菌根大型真菌中，初级碳水化合物代谢在碳氮交换和子实体发育的代谢支持中起着特殊的作用。在这项研究中，我们利用从最近测序的黑孢块茎基因组中检索到的信息，结合转录组分析数据和靶向验证实验，构建了植物共生子囊菌中支持碳水化合物代谢的蛋白质的第一个全基因组目录。对糖酵解、戊糖磷酸、三羧酸、乙醛酸盐和甲基柠檬酸途径、糖原、海藻糖和甘露醇代谢和细胞壁前体等酶的100多个基因进行了注释。利用全基因组寡阵列表达数据和所选基因的实时逆转录-聚合酶链反应分析，研究了这些途径在黑孢霉生命周期不同阶段的转录调控。最重要的结果是鉴定了甲基柠檬酸循环基因和酸转化酶，这是在植物共生丝状真菌中首次描述的这种酶。编码海藻糖、乙醛酸盐和甲基柠檬酸盐酶的转录本子集在子实体中表达上调，而涉及甘露醇和糖原代谢的基因分别在菌丝和外生菌根中优先表达。这些数据表明黑孢霉碳水化合物代谢的特定分支具有高度的生命周期阶段特化。
P>Primary carbohydrate metabolism plays a special role related to carbon/nitrogen exchange, as well as metabolic support of fruiting body development, in ectomycorrhizal macrofungi. In this study, we used information retrieved from the recently sequenced Tuber melanosporum genome, together with transcriptome analysis data and targeted validation experiments, to construct the first genome-wide catalogue of the proteins supporting carbohydrate metabolism in a plant-symbiotic ascomycete.More than 100 genes coding for enzymes of the glycolysis, pentose phosphate, tricarboxylic acid, glyoxylate and methylcitrate pathways, glycogen, trehalose and mannitol metabolism and cell wall precursor were annotated. Transcriptional regulation of these pathways in different stages of the T. melanosporum lifecycle was investigated using whole-genome oligoarray expression data together with real-time reverse transcription-polymerase chain reaction analysis of selected genes.The most significant results were the identification of methylcitrate cycle genes and of an acid invertase, the first enzyme of this kind to be described in a plant-symbiotic filamentous fungus.A subset of transcripts coding for trehalose, glyoxylate and methylcitrate enzymes was up-regulated in fruiting bodies, whereas genes involved in mannitol and glycogen metabolism were preferentially expressed in mycelia and ectomycorrhizas, respectively. These data indicate a high degree of lifecycle stage specialization for particular branches of carbohydrate metabolism in T. melanosporum.